多能干细胞与羊膜上皮相互作用对人胚胎发生的体外研究。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-07-03 DOI:10.1007/7651_2025_643

Daniela Ávila-González, Axel Castro-Abrego, Jonathan Salazar-Alonso, Omar Martínez Alarcon, Alejandro Martinez-Juarez, Anayansi Molina Hernández, Nestor Emmanuel Diaz-Martinez, Wendy Portillo, Nestor Fabián Diaz

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引用次数: 0

摘要

在灵长类胚胎的着床期，外胚层与羊膜上皮共存，表明该组织在胚胎发生中起关键作用。由于其对胚胎模式和组织规范的潜在影响，这种相互作用引起了发育生物学和再生医学的极大兴趣。在这项研究中，我们研究了人羊膜上皮细胞（hAEC）与人胚胎干细胞（hESC）共培养是否能增强后者向人原始生殖细胞样细胞（hPGCLC）分化的能力。

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Interaction Between Pluripotent Stem Cells and Amniotic Epithelium for the In Vitro Study of Human Embryogenesis.

During the peri-implantation stage in primate embryos, the epiblast coexists with amniotic epithelium, suggesting a pivotal role for this tissue in embryogenesis. This interaction has attracted considerable interest in developmental biology and regenerative medicine due to its potential influence on embryonic patterning and tissue specification. In this protocol, we examined whether co-culturing human amniotic epithelial cells (hAEC) with human embryonic stem cells (hESC) enhances the latter's capacity for differentiation toward human primordial germ cell-like cells (hPGCLC).

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.