犬巴贝斯虫病原生寄生虫巴贝斯虫的基因组组装与注释。

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Neelam Redekar, Xu Wang, Luis Neves, Steven Brooks, Justin Lack, Andrew Leisewitz, Hans Ackerman
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引用次数: 0

摘要

背景:顶复体寄生虫,罗西巴贝斯虫,是一种易变蜱传播的病原体,引起最严重形式的犬巴贝斯虫病。与狗的其他巴贝斯虫病原体相比,罗西贝斯虫表现出独特的病理生理、毒力和对药物的反应,这与小型巴贝斯虫寄生虫不同。结果:在这里,我们报道了从南非彼得马里茨堡的一只病狗中分离到的罗西巴贝斯虫菌株PMB -的第一个接近完整的染色体水平组装。利用长读HiFi数据进行组装,获得了21.06 Mbp的基因组大小,跨越了5个基因密集的染色体水平支架,一个顶体支架和其余54个未放置的低基因密度支架,N50为1.32 Mb, BUSCO顶复合体完整性为96.6%。基因组注释共鉴定出3098个蛋白质编码基因、71个tRNA和16个rRNA基因。线粒体基因组(6.4 Kbp)也被鉴定出来。此外,还报道了另外两个田间菌株的基因组组装。比较基因组分析结果显示,3个分离株存在4个基因组反转和多个多态性,但基因组内SNP和indel密度较高。尽管存在这些差异,三株罗西杆菌的基因组组装显示99%保守的同源基因集。罗西巴贝斯虫分离株PMB蛋白编码基因与其他4种巴贝斯虫共有约76%。结论:该报告提供了有价值的基因组信息,对了解犬巴贝斯虫病的罗氏杆菌进化、毒力和潜在的药物靶点至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis.

Background: Apicomplexan parasite, Babesia rossi, is an Ixodid tick-transmitted pathogen that causes the most severe form of canine babesiosis disease. Compared to other Babesia pathogens of dogs, B. rossi exhibits unique pathophysiology, virulence, and a responsiveness to drugs that differs from the small Babesia parasites.

Results: Here we report the first near-complete chromosome-level assembly of Babesia rossi strain PMB - isolated from a sick dog from Pietermaritzburg, South Africa. Assembly with long-read HiFi data yielded 21.06 Mbp genome size, spanning across five gene-dense chromosome-level scaffolds, a single apicoplast scaffold, and a remaining 54 unplaced low gene density scaffolds with 1.32 Mb N50 and 96.6% BUSCO Apicomplexan completeness. The genome annotation identified a total of 3,098 protein-coding genes, 71 tRNA, and 16 rRNA genes. The mitochondrial genome (6.4 Kbp) was also identified. Genome assemblies of two additional field isolates of B. rossi were also reported. Comparative genomic analyses revealed four syntenic genomic inversions and multiple polymorphisms across three B. rossi isolates, although SNP and indel density was higher within the gene desserts of the genomes. Despite these differences, three B. rossi isolates' genome assemblies showed 99% conserved orthologous gene sets. About 76% of protein-coding genes of Babesia rossi isolate PMB were shared with four other Babesia species.

Conclusion: This report provides valuable genomic information that is crucial to comprehend B. rossi evolution, virulence, and potential drug targets for canine babesiosis.

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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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