油菜素内酯介导的类胡萝卜素调节增强辣椒的抗寒性。

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jie Li, Jian Li, Jianghong Luo, Juan Li, Hamza Sohail, Keyan Zhu, Ping Yang
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引用次数: 0

摘要

背景:低温胁迫严重限制了辣椒(Capsicum annuum L.)的生长发育。油菜素内酯(BRs)和类胡萝卜素在缓解低温胁迫中起关键作用。目前,关于BRs与类胡萝卜素调控关系的研究还比较有限。本研究利用病毒诱导基因沉默(vigus)技术,对BR信号通路关键转录因子CaBZR1 (brassinazol - resistant 1)和类胡萝卜素合成基因CaLCYB(番茄红素β-环化酶)进行了沉默。探讨了br介导的类胡萝卜素对抗寒性的调节作用。结果:结果表明,分别沉默CaBZR1和CaLCYB后,类胡萝卜素含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)等抗氧化酶活性以及叶绿素荧光参数(Fv/Fm)均显著降低。超氧阴离子(O₂⁻)、过氧化氢(H₂O₂)和丙二醛(MDA)的水平显著增加,导致膜脂的氧化损伤。冷应答基因CaFbox1 (F-box蛋白1)、CaMBF1(多蛋白桥接因子1)和CaNAC3(含NAC结构域蛋白3)的表达显著降低。外源喷施EBR减轻了低温胁迫下CaBZR1和calcyb沉默植株的损伤。结论:这些结果强调了类胡萝卜素和BRs通过提高抗氧化酶活性、清除活性氧(ROS)和转录反应来增强辣椒的抗寒能力。该研究为低温胁迫下BR信号与类胡萝卜素代谢的相互作用提供了新的见解,并为提高作物的低温耐受性提供了潜在的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Brassinosteroid-mediated carotenoid regulation enhances chilling tolerance in pepper.

Background: Low temperature stress severely limits the growth and development of pepper (Capsicum annuum L.). Brassinolides (BRs) and carotenoids play key roles in alleviating low-temperature stress. At present, the research on the regulatory relationship between BRs and carotenoids remains rather limited. In this study, CaBZR1 (Brassinazole-Resistant 1), a key transcription factor of BR signaling pathway, and CaLCYB (Lycopene β-Cyclase), a carotenoid synthesis gene, were silenced using virus-induced gene silencing (VIGS) technology. The role of BR-mediated carotenoid regulation of cold tolerance was explored.

Results: The results showed that after silencing CaBZR1 and CaLCYB respectively, the carotenoid content, the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), as well as the chlorophyll fluorescence parameter (Fv/Fm) all decreased significantly. The levels of superoxide anion (O₂⁻), hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) increased significantly, leading to oxidative damage to membrane lipids. The expressions of cold-responsive genes such as CaFbox1 (F-box protein 1), CaMBF1 (Multiprotein Bridging Factor 1) and CaNAC3 (NAC domain containing protein 3) were significantly reduced. Exogenous spraying of EBR alleviated the damage in CaBZR1 and CaLCYB-silenced plants under low-temperature stress.

Conclusions: These results emphasize that carotenoids and BRs enhance cold resistance in pepper by enhancing antioxidant enzyme activities, scavenging reactive oxygen species (ROS) and transcriptional responses. This study provides new insights into the interactions between BR signaling and carotenoid metabolism under low-temperature stress and offers a potential strategy for improving low-temperature tolerance in crops.

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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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