Irina Muntaabski, Ricardo Salvador, Romina M Russo, Juan P Wulff, Lucas Landi, María C Liendo, Silvia B Lanzavecchia, Alejandra C Scannapieco
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引用次数: 0
摘要
外寄生瓦螨是西方蜜蜂的主要全球威胁。对以杀螨剂为基础的治疗方法日益增长的耐药性激发了人们对替代控制策略的兴趣。在这项研究中,我们采用了一种新颖高效的dsRNA递送方法来探索基于RNA干扰(RNAi)的方法在蜜蜂群体中控制瓦螨的潜力。通过半田间实验,研究了沉默6个靶基因(ptch1、ap-1、larp6、chisal、vg1和vg6)对螨死亡率和繁殖的影响。基因表达分析显示,与dsGFP对照相比,dsRNA处理的螨虫转录物水平显著降低,敲除效率从88.6%到97.2%不等。ptch1、ap-1和vg1基因的沉默导致螨虫不育的显著增加,这与它们在卵母细胞成熟和胚胎发生中的已知作用一致。此外,先前被描述为有效喂养瓦螨所必需的凿凿的沉默导致螨虫死亡率显着增加。这些结果突出了基于rnai的Varroa控制策略的有希望的基因靶点。此外,我们的研究提供了新的见解,包括Wnt, c-Jun n -末端激酶,Hedgehog和凋亡参与螨虫繁殖和生存的分子途径,为开发更有效的生物技术控制工具铺平了道路。
Assessing the role of key genes involved in the reproductive success of the honey bee parasite Varroa destructor.
The ectoparasite Varroa destructor is the primary global threat to the western honey bee, Apis mellifera. Growing resistance to acaricide-based treatments has spurred interest in alternative control strategies. In this study, we employed a novel and efficient dsRNA delivery method to explore the potential of RNA interference (RNAi)-based approaches for Varroa control in honey bee colonies. We assessed the effects of silencing six target genes (ptch1, ap-1, larp6, chisal, vg1, and vg6) on mite mortality and reproduction through a semi-field experiment. Gene expression analysis revealed significantly reduced transcript levels in mites treated with dsRNA compared to dsGFP controls, with knockdown efficiencies ranging from 88.6% to 97.2%. Silencing of ptch1, ap-1, and vg1 genes resulted in a significant increase in mite infertility, aligning with their known roles in oocyte maturation and embryogenesis. Additionally, silencing of chisal, previously described as essential for effective Varroa feeding, led to a marked increase in mite mortality. These results highlight promising gene targets for RNAi-based Varroa control strategies. Furthermore, our study provides new insights into the molecular pathways involved in mite reproduction and survival, including Wnt, c-Jun N-terminal kinase, Hedgehog, and apoptosis, paving the way for the development of more effective biotechnological control tools.
期刊介绍:
BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics.
BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.