Gladys Kaba, Andrew Stevenson, Samuel A Sakyi, Thomas O Konney, Nicholas A Titiloye, Samuel A Oppong, Kwabena Amo-Antwi, Francis Agyemang-Yeboah, Kate Cuschieri, Sheila V Graham
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引用次数: 0
摘要
目的：加纳HPV-18和HPV-45谱系/亚谱系变异的E6和E7 DNA序列谱。设计：横断面研究。机构：库马西Komfo Anokye教学医院产科/妇科理事会以及阿克拉Korle-Bu教学医院放射治疗/核医学部和计划生育科。参与者：207例临床怀疑宫颈癌（CxCa）或确诊的CxCa/癌前病例。方法：在检测24种hpv基因型的207份样本中，从2018年10月至2020年11月收集HPV-18（40份样本）和/或HPV-45（28份样本）L1 DNA阳性的个体的宫颈拭子。从方便样本和HPV-E6/E7-PCR （33/40-HPV-18-+ve或20/28-HPV-45-+ve样品）中提取DNA，进行测序和BLAST分析。结果：经PCR扩增，33份（HPV-18+ve）样本中26份E6/E7基因区和20份（HPV-45+ve）样本中10份（10份）符合测序条件。对于HPV-18变体，26个样本中有24个（92.31%）为谱系- b /C，包括谱系-C样本和22个谱系- b样本（其中10个样本为E7-SNP-C665T）。10个HPV-45变体中有9个是亚谱系a1，其中2个样本同时含有e6 - snp - c134t和C4I7T，其中一个样本同时检测到e6 - snp - c134t、G415C和C4I7T。结论：我们的研究证实了hpv -45-亚谱系- a1和hpv -18-谱系- b在加纳占主导地位（非洲特异性hpv -18-谱系- c很少发生）。我们的研究提供了加纳CxCa病例中HPV-18和HPV-45谱系和亚谱系的E6/E7 snp的初步数据。我们希望我们的数据将为未来HPV类型特异性核苷酸变化的模式和分布的研究提供信息，从而对治疗干预有用。资助：英国政府（GK）英联邦分地奖学金（含研究支持资助），编号GHCN201823。GK还获得加纳公立大学高级成员图书和研究津贴，用于在加纳开展研究活动。
Analysis of Human Papillomavirus-HPV-18 and HPV-45 Type-specific variants in a contemporary cohort of individuals with cervical disease in Ghana.
Objectives: E6 and E7 DNA sequence profile of HPV-18 and HPV-45 lineage/sublineage variants in Ghana.
Design: A cross-sectional study.
Setting: Obstetrics/Gynaecology Directorate, Komfo Anokye Teaching Hospital, Kumasi and Department of Radiotherapy/Nuclear Medicine and the Family Planning Unit, Korle-Bu Teaching Hospital, Accra.
Participants: 207 individuals referred with clinical suspicion of cervical cancer (CxCa) or confirmed CxCa/precancer cases.
Methods: Cervical swabs were collected (from October 2018 to November 2020) from individuals, with L1 DNA positivity for HPV-18(40 samples) and/or HPV-45(28 samples), out of 207 samples tested for 24-HPV-genotypes. DNA was extracted from a convenience sample and HPV-E6/E7-PCR (33/40-HPV-18-+ve- or 20/28-HPV-45-+ve samples), sequencing, and BLAST analysis was carried out.
Results: After PCR amplification, the E6/E7 gene regions of 26 out of 33(HPV-18+ve) samples and ten (10) out of 20 (HPV-45+ve) samples were eligible for sequencing. For HPV-18 variants, 24 out of 26 samples (92.31%) were of lineage-B/C, including samples of lineage-C and 22 samples of lineage-B (out of which ten (10) samples were with E7-SNP-C665T). Nine out of ten HPV-45 variants were sublineage-A1, of which two (2) samples harboured both E6-SNPs-C134T and C4I7T including one sample with E6-SNPs-C134T, G415C, C4I7T detected together.
Conclusions: Our study confirms a dominance of HPV-45-sublineage-A1 and HPV-18-lineage-B (with rare occurrence of Africa-specific HPV-18-lineage-C) variants in Ghana. Our study provides preliminary data on E6/E7 SNPs of HPV-18 and HPV-45 lineages and sublineages among CxCa cases in Ghana. We hope our data will inform future studies on pattern and distribution of HPV type-specific nucleotide changes that can be useful for therapeutic intervention.
Funding: UK Government, (GK) Commonwealth Split-Site Scholarship with research support grant, number GHCN201823. GK also received Ghana public universities senior members book & research allowance, for research activities carried out in Ghana.
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