工程，设计和选择荧光激活蛋白的先进成像和生物传感。

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Protein Engineering Design & Selection Pub Date : 2025-01-10 DOI:10.1093/protein/gzaf007

Lina El Hajji, Arnaud Gautier

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引用次数: 0

摘要

荧光激活蛋白（FAP）已成为一类新的基于荧光蛋白成像的遗传编码工具，补充了现有的由荧光蛋白和自标记标签组成的工具包。FAP有能力结合并激活小分子的荧光，这些小分子被称为荧光原，否则它们就不会发出荧光，从而使蛋白质定位具有高特异性和很少的背景。在这篇综述中，我们介绍了FAP和基于FAP的各种蛋白质支架报告的工程，重点介绍了设计和工程其特性用于特定生物成像应用的不同策略。

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Engineering, design and selection of fluorescence-activating proteins for advanced imaging and biosensing.

Fluorescence-activating proteins (FAP) have emerged as a novel class of genetically encoded tools for fluorescence-based protein imaging, complementing the existing toolkit consisting of fluorescent proteins and self-labeling tags. FAP have the ability to bind and activate the fluorescence of small molecules, called fluorogens, that are otherwise non-fluorescent, allowing protein localization with high specificity and little background. In this review, we present the engineering of FAP and FAP-based reporters from various protein scaffolds, focusing on the different strategies implemented to design and engineer their properties for specific biological imaging applications.

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来源期刊

Protein Engineering Design & Selection 生物-生化与分子生物学

CiteScore

3.30

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Protein Engineering, Design and Selection (PEDS) publishes high-quality research papers and review articles relevant to the engineering, design and selection of proteins for use in biotechnology and therapy, and for understanding the fundamental link between protein sequence, structure, dynamics, function, and evolution.