生物启发纳米微凝胶微球通过微环境的协同多靶点重塑治疗牙周炎。

IF 12.4 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Theranostics Pub Date : 2025-06-09 eCollection Date: 2025-01-01 DOI:10.7150/thno.112782
Siqi Zhou, Yuxin Zhang, Liwen Zheng, Chang Liu, Jiajun Chen, Yaxian Liu, Shidian Ran, Tong-Chuan He, Mengqin Gu, Si Wu, Fugui Zhang, Hongmei Zhang
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引用次数: 0

摘要

背景:牙周炎是一种常见的口腔炎症性疾病,可导致牙槽骨吸收和牙齿脱落。难以控制,易复发。目前的治疗常常因牙周袋深而弯曲和耐抗生素细菌(如核梭杆菌)而达不到目的。值得注意的是,目前的临床治疗无法同时满足三个关键目标:强大的抗菌功效,有效的抗炎活性和有效的牙周再生能力。方法:受莲花结构的启发,制备纳米/微米复合水凝胶微球(PDA/BBR@Gel@BMP9-PDLSC),包封聚多巴胺(PDA)纳米颗粒,携带小檗碱(PDA/BBR)和bmp9感染的pdlsc (BMP9-PDLSC)。结果:微球具有良好的生物相容性和持续的药物释放,并具有显著的抗菌、抗炎和骨组织再生作用。体内研究证实了其治疗颅骨缺损和牙周炎伴持续性核仁梭菌感染的有效性,显示出优越的新骨形成和抗炎作用,无器官毒性。值得注意的是,我们的研究表明,抗炎和成骨作用是由于PDA/BBR@Gel@BMP9-PDLSC微球释放的BBR和BMP9的协同作用。RNA-Seq和Western blot分析显示,BBR和BMP9通过调节参与TNF、TGF-β和PPAR信号通路的关键基因,协同减少炎症,促进骨形成。结论:本研究为抗菌、抗炎、骨组织再生综合治疗牙周炎提供了新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioinspired nano-micron hydrogel microspheres for periodontitis therapy through synergistic multi-targeted remodeling of microenvironment.

Background: Periodontitis is a prevalent oral inflammatory disease that leads to alveolar bone resorption and tooth loss. It is hard to control and prone to recurrence. Current treatments often fall short due to deep, tortuous periodontal pockets and antibiotic-resistant bacteria, such as Fusobacterium nucleatum (F. nucleatum). Notably, current clinical therapies fail to simultaneously fulfill three critical objectives: robust antimicrobial efficacy, potent anti-inflammatory activity, and effective periodontal regenerative capacity. Methods: Inspired by the structure of the lotus flower, nano/micron-combined hydrogel microspheres (PDA/BBR@Gel@BMP9-PDLSC), encapsulating polydopamine (PDA) nanoparticles carrying berberine (PDA/BBR) and BMP9-infected PDLSCs (BMP9-PDLSC), were developed. Results: Microspheres exhibited excellent biocompatibility and sustained drug release, along with significant antibacterial, anti-inflammatory, and bone tissue regenerative effects. In vivo studies confirmed their efficacy in treating calvaria defects and periodontitis with persistent F. nucleatum infection, showing superior new bone formation and anti-inflammatory effects without organ toxicity. Notably, our study demonstrated that the anti-inflammation and osteogenesis effects were due to the synergistic effects of BBR and BMP9 released by PDA/BBR@Gel@BMP9-PDLSC microspheres. RNA-Seq and Western blot analysis showed that BBR and BMP9 synergistically reduced inflammation and promoted bone formation by regulating key genes involved in TNF, TGF-β, and PPAR signaling pathways. Conclusions: Our study provides a novel approach for comprehensively treating periodontitis with antibacterial, anti-inflammatory, and bone tissue regenerative effects.

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来源期刊
Theranostics
Theranostics MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
25.40
自引率
1.60%
发文量
433
审稿时长
1 months
期刊介绍: Theranostics serves as a pivotal platform for the exchange of clinical and scientific insights within the diagnostic and therapeutic molecular and nanomedicine community, along with allied professions engaged in integrating molecular imaging and therapy. As a multidisciplinary journal, Theranostics showcases innovative research articles spanning fields such as in vitro diagnostics and prognostics, in vivo molecular imaging, molecular therapeutics, image-guided therapy, biosensor technology, nanobiosensors, bioelectronics, system biology, translational medicine, point-of-care applications, and personalized medicine. Encouraging a broad spectrum of biomedical research with potential theranostic applications, the journal rigorously peer-reviews primary research, alongside publishing reviews, news, and commentary that aim to bridge the gap between the laboratory, clinic, and biotechnology industries.
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