烟草NAC家族的全基因组鉴定及NtNAC236基因的功能鉴定。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-06-27 DOI:10.1016/j.ygeno.2025.111072

Tenghang Xu , Jie Liu , Hailan Liu , Jie Zhang , Linggai Cao , Zhixiao Yang , Rengang Wang , Zhenqing Guo , Shizhou Yu

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引用次数: 0

摘要

干旱胁迫严重限制了作物的生长，NAC转录因子（TFs）在干旱响应中起着至关重要的作用。在烟草中鉴定出266个NAC基因，通过系统发育和表达分析发现NtNAC236是关键的干旱响应TF。在K326中过表达NtNAC236增强了其耐旱性，亚细胞定位证实了其核作用。对NtNAC236- oe系和WT植株的转录组和DAP-Seq分析显示，NtNAC236在干旱条件下调控458个差异表达的靶基因，这些基因在胁迫响应途径中富集。一个共表达网络进一步将NtNAC236与43个干旱相关基因联系起来。我们的研究结果确定了NtNAC236是一个关键的抗旱调节剂，并为提高作物耐旱性的机制研究提供了基础。

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Genome-wide identification of NAC family and functional characterization of NtNAC236 in tobacco (Nicotiana tabacum)

Drought stress severely limits crop growth, and NAC transcription factors (TFs) are critical for drought response. In Nicotiana tabacum, we identified 266 NAC genes, among which NtNAC236 emerged as a key drought-responsive TF based on phylogenetic and expression analyses. Overexpressing NtNAC236 in K326 enhanced its drought tolerance, and subcellular localization confirmed its nuclear role. Transcriptome and DAP-Seq analyses of NtNAC236-OE lines and WT plants revealed that NtNAC236 regulates 458 differentially expressed target genes under drought, enriched in stress-response pathways. A co-expression network further linked NtNAC236 to 43 drought-associated genes. Our findings establish NtNAC236 as a pivotal drought-resistance regulator and provide a foundation for mechanistic studies to improve crop drought tolerance.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.