Eun-Yeong Bok , Won-Jae Lee , HyeonJeong Lee , Chan-Hee Jo , Chae-Yeon Hong , Seo-yoon Kang , Sanghyeon Park , Tae-Sung Hwang , Jaemin Kim , Sung-Lim Lee , Yong-Ho Choe , Sang-Il Lee
{"title":"类风湿关节炎中胚胎干细胞衍生间充质干细胞的免疫调节、抗滑膜细胞和抗破骨能力。","authors":"Eun-Yeong Bok , Won-Jae Lee , HyeonJeong Lee , Chan-Hee Jo , Chae-Yeon Hong , Seo-yoon Kang , Sanghyeon Park , Tae-Sung Hwang , Jaemin Kim , Sung-Lim Lee , Yong-Ho Choe , Sang-Il Lee","doi":"10.1016/j.yexcr.2025.114660","DOIUrl":null,"url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, fibroblast-like synoviocyte hyperactivation, and osteoclast-mediated bone destruction. Mesenchymal stem cells (MSCs) derived from adult tissues exhibit therapeutic potential owing to their regenerative and immunomodulatory properties; However, commercialization remains challenging due to limitations in large-scale production with consistent quality. Contrastingly, MSCs derived from embryonic stem cells can be continuously produced and exhibit minimal variation, with high biological and genetic uniformity. We confirmed that DW-MSCs exhibit MSC characteristics, including proliferative capacity, surface marker expression, and immunomodulatory function in vitro, by comparison with umbilical cord-derived MSCs (UC-MSCs) as one representative type of adult tissue-derived MSCs. We further evaluated their effects on RA pathology via in vitro assays of FLS migration and osteoclastogenesis. Although DW-MSCs showed lower immunosuppressive capacity than UC-MSCs, DW-MSCs retained functional immunosuppressive efficacy and exhibited superior inhibition of FLS migration and osteoclastogenesis, both of which are critical in the complex pathogenesis of RA. In a collagen-induced arthritis mouse model, intra-articular injection of DW-MSCs reduced inflammation and bone erosion, with an increased regulatory T cell/T-helper-17 ratio in draining lymph nodes. These findings demonstrated the therapeutic potential of DW-MSCs and support their application as a scalable and standardized stem cell-based treatment for RA.</div></div>","PeriodicalId":12227,"journal":{"name":"Experimental cell research","volume":"450 2","pages":"Article 114660"},"PeriodicalIF":3.5000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunomodulatory, anti-synoviocyte, and anti-osteoclastic abilities of embryonic stem cell-derived mesenchymal stem cells in rheumatoid arthritis\",\"authors\":\"Eun-Yeong Bok , Won-Jae Lee , HyeonJeong Lee , Chan-Hee Jo , Chae-Yeon Hong , Seo-yoon Kang , Sanghyeon Park , Tae-Sung Hwang , Jaemin Kim , Sung-Lim Lee , Yong-Ho Choe , Sang-Il Lee\",\"doi\":\"10.1016/j.yexcr.2025.114660\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, fibroblast-like synoviocyte hyperactivation, and osteoclast-mediated bone destruction. Mesenchymal stem cells (MSCs) derived from adult tissues exhibit therapeutic potential owing to their regenerative and immunomodulatory properties; However, commercialization remains challenging due to limitations in large-scale production with consistent quality. Contrastingly, MSCs derived from embryonic stem cells can be continuously produced and exhibit minimal variation, with high biological and genetic uniformity. We confirmed that DW-MSCs exhibit MSC characteristics, including proliferative capacity, surface marker expression, and immunomodulatory function in vitro, by comparison with umbilical cord-derived MSCs (UC-MSCs) as one representative type of adult tissue-derived MSCs. We further evaluated their effects on RA pathology via in vitro assays of FLS migration and osteoclastogenesis. Although DW-MSCs showed lower immunosuppressive capacity than UC-MSCs, DW-MSCs retained functional immunosuppressive efficacy and exhibited superior inhibition of FLS migration and osteoclastogenesis, both of which are critical in the complex pathogenesis of RA. In a collagen-induced arthritis mouse model, intra-articular injection of DW-MSCs reduced inflammation and bone erosion, with an increased regulatory T cell/T-helper-17 ratio in draining lymph nodes. These findings demonstrated the therapeutic potential of DW-MSCs and support their application as a scalable and standardized stem cell-based treatment for RA.</div></div>\",\"PeriodicalId\":12227,\"journal\":{\"name\":\"Experimental cell research\",\"volume\":\"450 2\",\"pages\":\"Article 114660\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental cell research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014482725002605\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014482725002605","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Immunomodulatory, anti-synoviocyte, and anti-osteoclastic abilities of embryonic stem cell-derived mesenchymal stem cells in rheumatoid arthritis
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, fibroblast-like synoviocyte hyperactivation, and osteoclast-mediated bone destruction. Mesenchymal stem cells (MSCs) derived from adult tissues exhibit therapeutic potential owing to their regenerative and immunomodulatory properties; However, commercialization remains challenging due to limitations in large-scale production with consistent quality. Contrastingly, MSCs derived from embryonic stem cells can be continuously produced and exhibit minimal variation, with high biological and genetic uniformity. We confirmed that DW-MSCs exhibit MSC characteristics, including proliferative capacity, surface marker expression, and immunomodulatory function in vitro, by comparison with umbilical cord-derived MSCs (UC-MSCs) as one representative type of adult tissue-derived MSCs. We further evaluated their effects on RA pathology via in vitro assays of FLS migration and osteoclastogenesis. Although DW-MSCs showed lower immunosuppressive capacity than UC-MSCs, DW-MSCs retained functional immunosuppressive efficacy and exhibited superior inhibition of FLS migration and osteoclastogenesis, both of which are critical in the complex pathogenesis of RA. In a collagen-induced arthritis mouse model, intra-articular injection of DW-MSCs reduced inflammation and bone erosion, with an increased regulatory T cell/T-helper-17 ratio in draining lymph nodes. These findings demonstrated the therapeutic potential of DW-MSCs and support their application as a scalable and standardized stem cell-based treatment for RA.
期刊介绍:
Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.