Luciana Almeida, Elena Fajardo-Ruiz, Sophie Brameyer, Kirsten Jung
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The Framework for Genetic Engineering in Desulfovibrionaceae.
Desulfovibrionaceae are sulfate-reducing bacteria that are ubiquitous in anoxic environments and are also found in the human gastrointestinal tract. Their numbers are low in a healthy gut, but various intestinal and extraintestinal diseases are associated with a massive proliferation of Desulfovibrio spp., leading to an increase in toxic hydrogen sulfide, the product of dissimilatory sulfate respiration. The physiological and signaling capabilities of intestinal Desulfovibrio spp. are virtually unknown. To explore them, an efficient platform for targeted genetic engineering of Desulfovibrio spp. is required. In this review, we provide an overview of the available genetic parts and techniques for establishing efficient and precise genetic manipulation systems in Desulfovibrionaceae and discuss the challenges.
期刊介绍：
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.
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