{"title":"靶向肺癌上皮细胞向间质转化的苯并恶嗪类抑制剂的研究进展。","authors":"Naphatson Phansom, Zin Zin Ei, Bhurichaya Innets, Worawat Wattanathana, Theera Rittirod, Acharawan Thongmee, Keerati Joyjamras, Pithi Chanvorachote","doi":"10.21873/anticanres.17649","DOIUrl":null,"url":null,"abstract":"<p><strong>Background/aim: </strong>The epithelial-to-mesenchymal transition (EMT) plays an essential role in lung cancer metastasis. This study aimed to explore the EMT inhibitory effect of the new compound 6,6'-(butylazanediyl) bis (methylene) bis (2,4-dimethylphenol) (2,4-diMBD).</p><p><strong>Materials and methods: </strong>Cell survival and proliferation were determined using cell viability and colony formation assays. Migration was analyzed using a wound-healing assay. The expression of proteins and mRNAs was examined using immunofluorescence assay and real-time quantitative PCR.</p><p><strong>Results: </strong>2,4-diMBD significantly suppressed colony formation and cell migration. In relation to its anti-migratory activity, we found that 2,4-diMBD decreased Snail, Slug, and zinc finger E-box binding homeobox (ZEB) levels at both the mRNA and protein levels, indicating EMT suppression in human lung cancer cells.</p><p><strong>Conclusion: </strong>2,4-diMBD exerts anti-metastatic properties in lung cancer cells <i>via</i> EMT inhibition, and is a promising compound for the treatment of lung cancer.</p>","PeriodicalId":8072,"journal":{"name":"Anticancer research","volume":"45 7","pages":"2807-2815"},"PeriodicalIF":1.7000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of a Benzoxazine-derived Inhibitor Targeting Epithelial-to-Mesenchymal Transition in Lung Cancer.\",\"authors\":\"Naphatson Phansom, Zin Zin Ei, Bhurichaya Innets, Worawat Wattanathana, Theera Rittirod, Acharawan Thongmee, Keerati Joyjamras, Pithi Chanvorachote\",\"doi\":\"10.21873/anticanres.17649\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background/aim: </strong>The epithelial-to-mesenchymal transition (EMT) plays an essential role in lung cancer metastasis. This study aimed to explore the EMT inhibitory effect of the new compound 6,6'-(butylazanediyl) bis (methylene) bis (2,4-dimethylphenol) (2,4-diMBD).</p><p><strong>Materials and methods: </strong>Cell survival and proliferation were determined using cell viability and colony formation assays. Migration was analyzed using a wound-healing assay. The expression of proteins and mRNAs was examined using immunofluorescence assay and real-time quantitative PCR.</p><p><strong>Results: </strong>2,4-diMBD significantly suppressed colony formation and cell migration. In relation to its anti-migratory activity, we found that 2,4-diMBD decreased Snail, Slug, and zinc finger E-box binding homeobox (ZEB) levels at both the mRNA and protein levels, indicating EMT suppression in human lung cancer cells.</p><p><strong>Conclusion: </strong>2,4-diMBD exerts anti-metastatic properties in lung cancer cells <i>via</i> EMT inhibition, and is a promising compound for the treatment of lung cancer.</p>\",\"PeriodicalId\":8072,\"journal\":{\"name\":\"Anticancer research\",\"volume\":\"45 7\",\"pages\":\"2807-2815\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anticancer research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21873/anticanres.17649\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anticancer research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21873/anticanres.17649","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
Development of a Benzoxazine-derived Inhibitor Targeting Epithelial-to-Mesenchymal Transition in Lung Cancer.
Background/aim: The epithelial-to-mesenchymal transition (EMT) plays an essential role in lung cancer metastasis. This study aimed to explore the EMT inhibitory effect of the new compound 6,6'-(butylazanediyl) bis (methylene) bis (2,4-dimethylphenol) (2,4-diMBD).
Materials and methods: Cell survival and proliferation were determined using cell viability and colony formation assays. Migration was analyzed using a wound-healing assay. The expression of proteins and mRNAs was examined using immunofluorescence assay and real-time quantitative PCR.
Results: 2,4-diMBD significantly suppressed colony formation and cell migration. In relation to its anti-migratory activity, we found that 2,4-diMBD decreased Snail, Slug, and zinc finger E-box binding homeobox (ZEB) levels at both the mRNA and protein levels, indicating EMT suppression in human lung cancer cells.
Conclusion: 2,4-diMBD exerts anti-metastatic properties in lung cancer cells via EMT inhibition, and is a promising compound for the treatment of lung cancer.
期刊介绍：
ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed.
ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies).
Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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