葡萄糖酸钠对成骨前细胞MC3T3-E1和去卵巢大鼠模型的抗骨质疏松活性。

IF 1.7 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Anqi Wang, Biyao Liu, Qingkai Zeng, Xiuhua Zhang, Yihong Xu, Xiao Zhang, Lei Chen, Juzheng Sheng, Huarong Shao, Fei Liu
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引用次数: 0

摘要

本研究旨在探讨葡萄糖醛酸钠(SG)对骨质疏松症(OP)的潜在治疗作用。为了达到这一目的,采用细胞计数试剂盒-8和碱性磷酸酶(ALP)活性测定法确定了SG刺激新生小鼠颅骨MC3T3-E1成骨细胞的最佳浓度。采用qRT-PCR和Western blotting分析成骨标志物。采用苏木精和伊红染色对胫骨组织进行病理形态学观察。采用酶联免疫吸附试验(ELISA)评估骨转换标志物(BTMs)水平。研究发现SG处理能有效促进MC3T3-E1细胞成骨分化和矿化,表现为ALP活性增强、钙沉积增加、关键成骨标志物如RUNX2、骨钙素和骨桥蛋白表达上调。此外,在去卵巢大鼠(绝经后OP模型)中,SG治疗显著促进骨形成,调节BTMs水平,增加骨矿物质密度。一致地,SG上调骨组织中成骨基因(RUNX2、OCN和OPN)的表达,进一步支持其成骨潜能。总的来说,这些发现表明SG具有刺激骨形成的能力,可能有望成为治疗OP的潜在药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-osteoporosis Activity of Sodium Glucuronate in Preosteo-blast MC3T3-E1 Cells and an Ovariectomized Rat Model.

The purpose of this study was to explore the potential therapeutic effect of sodium glucuronate (SG) on osteoporosis (OP). To achieve this aim, the optimal concentration of SG for stimulating MC3T3-E1 osteoblast cells derived from the calvaria of neonatal mice was determined using cell counting kit-8 and alkaline phosphatase (ALP) activity assays. Osteogenic markers were analyzed by qRT-PCR and Western blotting. The histopathological morphology of the tibial tissues was performed using hematoxylin and eosin staining. The levels of bone turnover markers (BTMs) were assessed using enzyme-linked immunosorbent assay (ELISA). SG treatment was found to effectively promote osteoblastic differentiation and mineralization in MC3T3-E1 cells, evidenced by enhanced ALP activity, increased calcium deposition, and upregulated expression of key osteogenic markers including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and osteopontin (OPN). Moreover, in ovariectomized rats, a model of postmenopausal OP, SG treatment significantly promoted bone formation, regulated the levels of BTMs, and augmented bone mineral density. Consistently, SG upregulated the expression of osteogenic genes (RUNX2, OCN, and OPN) in bone tissue, further supporting its osteogenic potential. Collectively, these findings suggest that SG possesses the ability to stimulate bone formation and may hold promise as a potential agent for the management of OP.

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来源期刊
CiteScore
3.50
自引率
5.00%
发文量
247
审稿时长
2 months
期刊介绍: Biological and Pharmaceutical Bulletin (Biol. Pharm. Bull.) began publication in 1978 as the Journal of Pharmacobio-Dynamics. It covers various biological topics in the pharmaceutical and health sciences. A fourth Society journal, the Journal of Health Science, was merged with Biol. Pharm. Bull. in 2012. The main aim of the Society’s journals is to advance the pharmaceutical sciences with research reports, information exchange, and high-quality discussion. The average review time for articles submitted to the journals is around one month for first decision. The complete texts of all of the Society’s journals can be freely accessed through J-STAGE. The Society’s editorial committee hopes that the content of its journals will be useful to your research, and also invites you to submit your own work to the journals.
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