三协同中空AuAg@CeO2等离子纳米酶通过智能手机集成的双模式生物传感实现快速碱性磷酸酶检测

IF 9.4 1区化学 Q1 CHEMISTRY, PHYSICAL

Journal of Colloid and Interface Science Pub Date : 2025-06-25 DOI:10.1016/j.jcis.2025.138272

Juan Tan , Shuaishuai Ding , Ling He , Xiu-wu Bian , Gan Tian

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引用次数: 0

摘要

碱性磷酸酶（ALP）是人体组织中的关键水解酶，是疾病诊断的关键生物标志物。在这里，我们报道了一种中空的AuAg@CeO2等离子纳米酶，通过三重协同催化用于ALP检测。分层设计的中空结构通过多尺度光散射增强了近红外（NIR）光子捕获，而AuAg-CeO2异质结则实现了定向电荷转移。alp介导的抗坏血酸生成触发级联机制：(1)等离子体热电子再生CeO2氧空位，优化H2O2吸附；(2)光热活化有利于H2O2解离；(3)局域表面等离子体共振（LSPR）放大界面电子动力学。这种协同作用增强了其过氧化物酶样活性，在10分钟内实现了纳摩尔ALP敏感性，比商业试剂盒提高了100倍。集成的基于智能手机的比色双模式分析能够以<； 10%的基质干扰交叉验证ALP定量，通过人体样品验证，回收率为94.8 - 105.4%。通过破译结构- lspr -活性相关性，这项工作开创了自适应纳米酶设计和精准医学智能诊断平台，弥合了护理点检测和早期疾病监测的关键差距。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Triple-synergistic hollow AuAg@CeO2 plasmonic nanozymes enable rapid alkaline phosphatase detection via smartphone-integrated dual-mode biosensing

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Triple-synergistic hollow AuAg@CeO2 plasmonic nanozymes enable rapid alkaline phosphatase detection via smartphone-integrated dual-mode biosensing

Alkaline phosphatase (ALP), a pivotal hydrolase in human tissues, serves as a key biomarker for disease diagnostics. Here, we report a hollow AuAg@CeO₂ plasmonic nanozyme engineered for ALP detection through triple synergistic catalysis. The hierarchically designed hollow architecture enhances near-infrared (NIR) photon capture via multiscale light scattering, while the AuAg-CeO₂ heterojunction enables directional charge transfer. ALP-mediated ascorbic acid generation triggers a cascade mechanism: (1) plasmonic hot electrons regenerate CeO₂ oxygen vacancies to optimize H₂O₂ adsorption; (2) photothermal activation facilitates H₂O₂ dissociation; and (3) localized surface plasmon resonance (LSPR) amplifies interfacial electron kinetics. This synergy boosts its peroxidase-like activity, achieving nanomolar ALP sensitivity—a 100-fold improvement over commercial kits within 10-min. Integrated smartphone-based colorimetric dual-mode analysis enables cross-validated ALP quantification with <10 % matrix interference, validated by human samples with 94.8–105.4 % recovery. By deciphering structure-LSPR-activity correlations, this work pioneers adaptive nanozyme design and intelligent diagnostic platforms for precision medicine, bridging critical gaps in point-of-care testing and early disease surveillance.

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来源期刊

Journal of Colloid and Interface Science 化学-物理化学

CiteScore

16.10

自引率

7.10%

发文量

2568

审稿时长

2 months

期刊介绍： The Journal of Colloid and Interface Science publishes original research findings on the fundamental principles of colloid and interface science, as well as innovative applications in various fields. The criteria for publication include impact, quality, novelty, and originality. Emphasis: The journal emphasizes fundamental scientific innovation within the following categories: A.Colloidal Materials and Nanomaterials B.Soft Colloidal and Self-Assembly Systems C.Adsorption, Catalysis, and Electrochemistry D.Interfacial Processes, Capillarity, and Wetting E.Biomaterials and Nanomedicine F.Energy Conversion and Storage, and Environmental Technologies