酶催化的模糊性

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-06-27 DOI:10.1016/j.sbi.2025.103106

Sachin S. Katti , Tvesha Parikh , Rachel J. Godek , Rebecca Page , Wolfgang Peti

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引用次数: 0

摘要

内在无序蛋白质/区域（IDPs/IDRs）经常参与动态电荷相互作用，通常被称为“模糊”相互作用。这些模糊的相互作用在酶调节和底物招募中起着关键作用，特别是对于蛋白激酶和蛋白磷酸酶。在这里，我们回顾了最近的进展，证明了激酶和磷酸酶及其同源调节剂和底物之间的分子间和分子内模糊相互作用如何允许酶组装，激活和底物募集。我们还强调了蛋白质抑制的独特机制，其中蛋白质磷酸酶通过与其活性位点金属的动态模糊相互作用被抑制。

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Fuzziness in enzymatic catalysis

Intrinsically disordered proteins/regions (IDPs/IDRs) frequently engage in dynamic charge:charge interactions, commonly referred to as ‘fuzzy’ interactions. These fuzzy interactions play critical roles in enzymatic regulation and substrate recruitment, especially for protein kinases and protein phosphatases. Here, we review recent advances that demonstrate how inter- and intramolecular fuzzy interactions among kinases and phosphatases and their cognate regulators and substrates allow for enzyme assembly, activation and substrate recruitment. We also highlight a unique mechanism of protein inhibition, where a protein phosphatase is inhibited by dynamic fuzzy interactions with its active site metals.

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation