蛋白酶催化水溶性低聚组氨酸的合成。

IF 1.3 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Industrial Biotechnology Pub Date : 2025-06-04 eCollection Date: 2025-06-01 DOI:10.1089/ind.2025.0010

Sarah P Black, Melinda Liu, Ke Xia, Sagar D Khare, Richard A Gross

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引用次数: 0

摘要

本文提出了一种“绿色”方法，通过蛋白酶催化肽合成水溶性低聚组氨酸肽。以木瓜蛋白酶和α-胰凝乳酶为催化剂，通过1h NMR实时监测合成过程。采用Pyrosetta计算模型计算木瓜蛋白酶和α-凝乳胰蛋白酶酰胺化与水解所需能量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Protease-Catalyzed Synthesis of Water-Soluble Oligohistidine.

This article presents a "green" approach to water-soluble oligohistidine peptides through protease-catalyzed peptide synthesis. Synthesis was catalyzed by papain and α-chymotrypsin, and reactions were monitored in-situ by real-time 1-hour NMR spectroscopy. Pyrosetta computational modeling was used to calculate the energy required for amidation versus hydrolysis with papain and α-chymotrypsin.

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来源期刊

Industrial Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

2.20

自引率

0.00%

发文量