环糊精包合络合离子辅助差示离子迁移率分离质谱法研究手性氨基酸对映体过量

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Simin Zhang, Xiangfeng Chen, T.-Y. Lui, Danna Hu, T.-W. Dominic Chan
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引用次数: 0

摘要

由于手性氨基酸具有相似的性质,开发一种快速、廉价、特异的手性氨基酸鉴定和分离方法具有挑战性。在captivesray - dms - ms系统中,通过对环糊精(CD)主-客体配合物的分析,建立了手性原子吸收光谱分离方法。与β-和γ- cd相比,α-CD具有最佳的分化性能。优化后的条件可在几分钟内对6对AA-α-CD配合物进行分析。利用该策略获得的离子图有效地确定了手性原子吸收剂的对映体比例,结果表明,l -丝氨酸和d -丝氨酸的摩尔比与峰丰度比之间具有很强的线性关系(R2 = 0.996)。该方法不需要使用金属离子,而且手性AA-α-CD络合离子离子图中的单峰形状简化了数据解释。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation on Chiral Amino Acids Enantiomeric Excess With Cyclodextrin Inclusion Complex Ions Assisted Differential Ion Mobility Separation Mass Spectrometry

Development of a rapid, inexpensive, and specific method for chiral amino acids (AAs) identification and separation is challenging owing to their similar properties. In this work, a chiral AAs separation method was developed by analyzing the AAs-cyclodextrin (CD) host–guest complex in the CaptiveSpray-DMS-MS system. Compared with β- and γ-CDs, α-CD was revealed to provide the best differentiation performance. The optimized conditions enabled the analysis of six pairs of respective AA-α-CD complexes within several minutes. Ionograms obtained using this strategy effectively determined the enantiomeric ratio of chiral AAs, as illustrated by the strong linearity (R2 = 0.996) between peak abundance ratios and molar ratios of L-serine and D-serine. This method does not require the use of metal ions, and the single peak shape in ionograms of chiral AA-α-CD complex ions simplifies data interpretation.

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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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