Simin Zhang, Xiangfeng Chen, T.-Y. Lui, Danna Hu, T.-W. Dominic Chan
{"title":"环糊精包合络合离子辅助差示离子迁移率分离质谱法研究手性氨基酸对映体过量","authors":"Simin Zhang, Xiangfeng Chen, T.-Y. Lui, Danna Hu, T.-W. Dominic Chan","doi":"10.1002/jms.5155","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Development of a rapid, inexpensive, and specific method for chiral amino acids (AAs) identification and separation is challenging owing to their similar properties. In this work, a chiral AAs separation method was developed by analyzing the AAs-cyclodextrin (CD) host–guest complex in the CaptiveSpray-DMS-MS system. Compared with β- and γ-CDs, <i>α</i>-CD was revealed to provide the best differentiation performance. The optimized conditions enabled the analysis of six pairs of respective AA-<i>α</i>-CD complexes within several minutes. Ionograms obtained using this strategy effectively determined the enantiomeric ratio of chiral AAs, as illustrated by the strong linearity (<i>R</i><sup>2</sup> = 0.996) between peak abundance ratios and molar ratios of L-serine and D-serine. This method does not require the use of metal ions, and the single peak shape in ionograms of chiral AA-α-CD complex ions simplifies data interpretation.</p>\n </div>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":"60 7","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation on Chiral Amino Acids Enantiomeric Excess With Cyclodextrin Inclusion Complex Ions Assisted Differential Ion Mobility Separation Mass Spectrometry\",\"authors\":\"Simin Zhang, Xiangfeng Chen, T.-Y. Lui, Danna Hu, T.-W. Dominic Chan\",\"doi\":\"10.1002/jms.5155\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Development of a rapid, inexpensive, and specific method for chiral amino acids (AAs) identification and separation is challenging owing to their similar properties. In this work, a chiral AAs separation method was developed by analyzing the AAs-cyclodextrin (CD) host–guest complex in the CaptiveSpray-DMS-MS system. Compared with β- and γ-CDs, <i>α</i>-CD was revealed to provide the best differentiation performance. The optimized conditions enabled the analysis of six pairs of respective AA-<i>α</i>-CD complexes within several minutes. Ionograms obtained using this strategy effectively determined the enantiomeric ratio of chiral AAs, as illustrated by the strong linearity (<i>R</i><sup>2</sup> = 0.996) between peak abundance ratios and molar ratios of L-serine and D-serine. This method does not require the use of metal ions, and the single peak shape in ionograms of chiral AA-α-CD complex ions simplifies data interpretation.</p>\\n </div>\",\"PeriodicalId\":16178,\"journal\":{\"name\":\"Journal of Mass Spectrometry\",\"volume\":\"60 7\",\"pages\":\"\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jms.5155\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jms.5155","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Investigation on Chiral Amino Acids Enantiomeric Excess With Cyclodextrin Inclusion Complex Ions Assisted Differential Ion Mobility Separation Mass Spectrometry
Development of a rapid, inexpensive, and specific method for chiral amino acids (AAs) identification and separation is challenging owing to their similar properties. In this work, a chiral AAs separation method was developed by analyzing the AAs-cyclodextrin (CD) host–guest complex in the CaptiveSpray-DMS-MS system. Compared with β- and γ-CDs, α-CD was revealed to provide the best differentiation performance. The optimized conditions enabled the analysis of six pairs of respective AA-α-CD complexes within several minutes. Ionograms obtained using this strategy effectively determined the enantiomeric ratio of chiral AAs, as illustrated by the strong linearity (R2 = 0.996) between peak abundance ratios and molar ratios of L-serine and D-serine. This method does not require the use of metal ions, and the single peak shape in ionograms of chiral AA-α-CD complex ions simplifies data interpretation.
期刊介绍:
The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions.
The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.