生物信息学分析确定ZBTB16是肺癌和泛癌的潜在免疫生物标志物。

IF 2.8 3区生物学 Q2 GENETICS & HEREDITY

Frontiers in Genetics Pub Date : 2025-06-09 eCollection Date: 2025-01-01 DOI:10.3389/fgene.2025.1541732

Danfei Shi, Yunxiang Cai, Di Zhu, Xinmin Li, Yong Li

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引用次数: 0

摘要

目的：肺癌（LC）是一种致命的癌症，也是一个具有挑战性的全球公共卫生问题。本研究的目的是利用生物信息学分析ZBTB16作为肺癌和各种其他癌症的免疫生物标志物的潜力。方法：从GSE3268、gse1987、GSE31547和GSE18842基因表达数据集中选取LC中重叠的差异表达基因（DEGs）。我们对这些差异表达基因进行了全面的生物信息学分析，旨在探索它们的富集功能和途径、相对表达水平、相互作用网络以及加权基因共表达网络（WGCNA）模块分析。验证ZBTB16在肺癌发生发展中的潜在作用，并进行免疫侵袭分析、泛癌分析和mRNA-miRNA联链分析。结果：16个基因表达升高，100个基因表达降低。其中，KEGG分析显示，这些deg显著参与补体和凝血级联，以及近端管状碳酸氢盐恢复等相关通路。既往研究表明，ZBTB16在多种全身性肿瘤中发挥重要作用，但其在肺癌中的功能尚未被揭示。WGCNA分析显示ZBTB16在肺癌中具有显著意义。因此，我们将重点关注ZBTB16。然后通过TCGA数据库、Human Protein Atlas数据库、全血qPCR检测验证ZBTB16在肺癌中的差异表达，并进行ZBTB16在肺癌中的免疫侵袭分析、泛癌分析、ZBTB16的mRNA miRNA连锁分析。结论：三种验证方法及泛癌分析均显示ZBTB16在肺癌及各种癌症中表达降低，可能是肺癌及各种癌症发生发展的关键基因。

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Bioinformatics analysis identifies ZBTB16 as a potential immune biomarker for lung cancer and pan-cancer.

Objective: Lung cancer (LC) is a deadly cancer and a challenging public health problem worldwide. The aim of this study is to use bioinformatics to analyze the potential of ZBTB16 as an immune biomarker in lung cancer and various other cancers.

Methods: Overlapping differentially expressed genes (DEGs) in LC were selected from GSE3268, GSE 1987, GSE31547 and GSE18842 gene expression data sets. We conducted a comprehensive bioinformatics analysis of these differentially expressed genes, aiming to explore their enrichment functions and pathways, relative expression levels, interaction networks, and weighted gene co expression network (WGCNA) module analysis. Then, the potential role of ZBTB16 in the occurrence and progression of lung cancer was verified, and immune invasion analysis, pan-cancer analysis and mRNA-miRNA link analysis were performed.

Results: There were 16 genes with increased expression and 100 genes with decreased expression. Among them, KEGG analysis showed that these DEGs were significantly involved in complement and coagulation cascades, as well as related pathways such as proximal tubular bicarbonate recovery. Previous studies have shown that ZBTB16 plays an important role in various systemic tumors, but its function in lung cancer has not been revealed. WGCNA analysis shows that ZBTB16 is significant in lung cancer. Therefore, we will focus our attention on ZBTB16. Then, TCGA database, Human Protein Atlas database, and whole blood qPCR testing were used to verify the differential expression of ZBTB16 in lung cancer, and immune invasion analysis of ZBTB16 in lung cancer, pan cancer analysis of ZBTB16, and mRNA miRNA linkage analysis of ZBTB16 were performed.

Conclusion: Three validation methods and pan cancer analysis all showed that the expression of ZBTB16 was reduced in lung cancer and various cancers, which may be a key gene for the occurrence and development of lung cancer and various cancers.

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来源期刊

Frontiers in Genetics Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

5.50

自引率

8.10%

发文量

3491

审稿时长

14 weeks

期刊介绍： Frontiers in Genetics publishes rigorously peer-reviewed research on genes and genomes relating to all the domains of life, from humans to plants to livestock and other model organisms. Led by an outstanding Editorial Board of the world’s leading experts, this multidisciplinary, open-access journal is at the forefront of communicating cutting-edge research to researchers, academics, clinicians, policy makers and the public. The study of inheritance and the impact of the genome on various biological processes is well documented. However, the majority of discoveries are still to come. A new era is seeing major developments in the function and variability of the genome, the use of genetic and genomic tools and the analysis of the genetic basis of various biological phenomena.