Omowunmi A Banjo, Abimbola O Adekanmbi, Oluwatimileyin J Akinbola, Benjamin T Thomas, Olusolape A F Ilusanya
{"title":"从医院废水中分离的多重耐药大肠杆菌质粒介导的喹诺酮类耐药基因的分子特征","authors":"Omowunmi A Banjo, Abimbola O Adekanmbi, Oluwatimileyin J Akinbola, Benjamin T Thomas, Olusolape A F Ilusanya","doi":"10.1177/11786302251342936","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>This study investigated the carriage of Plasmid-Mediated Quinolone Resistance (PMQR) genes in fluoroquinolone-resistant <i>Escherichia coli</i> recovered from wastewater generated by healthcare institutions.</p><p><strong>Materials and methods: </strong>Isolation of fluoroquinolone-resistant <i>Escherichia coli</i> was done on medium supplemented with 1 µg/mL of ciprofloxacin (a fluoroquinolone). Presumptive isolates were identified via the detection of <i>uidA</i> gene. Susceptibility of the isolates to a panel of antibiotics was done using disc diffusion method. Detection of PMQR genes in the isolates was done using primer-specific PCR.</p><p><strong>Results: </strong>Thirty fluoroquinolone-resistant <i>Escherichia coli</i> were obtained from the wastewater over a period of 6 months. The resistance to each of the antibiotic tested was: ampicillin (100%), ceftriaxone (100%), nalidixic acid (100%), tetracycline (96.7%), cefotaxime (96.7%), amoxicillin-clavulanate (80%), gentamicin (60%), cefoxitin (30%), and imipenem (3.3%). The Multiple Antibiotic Resistance Index (MARI) ranged from 0.6 to 0.9. The detection of PMQR genes in the 30 isolates was: <i>qnrA</i> (76.7%), <i>qnrB</i> (53.3%), <i>qnrS</i> (63.3%), <i>aac(6')-lb-cr</i> (43.3%), and <i>qepA</i> (43.3%). All the fluoroquinolone-resistant <i>Escherichia coli</i> carried at least one PMQR determinant.</p><p><strong>Conclusion: </strong>This study revealed that untreated hospital wastewaters are significant hub of multidrug-resistant and fluoroquinolone-resistant <i>Escherichia coli</i>, showing high carriage of PMQR genes, and may be a major contributor to the resistome of fluoroquinolone-resistant bacteria in the Nigerian environment.</p>","PeriodicalId":11827,"journal":{"name":"Environmental Health Insights","volume":"19 ","pages":"11786302251342936"},"PeriodicalIF":2.9000,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183395/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular Characterization of Plasmid-Mediated Quinolone Resistance Genes in Multidrug-Resistant <i>Escherichia coli</i> Isolated From Wastewater Generated From the Hospital Environment.\",\"authors\":\"Omowunmi A Banjo, Abimbola O Adekanmbi, Oluwatimileyin J Akinbola, Benjamin T Thomas, Olusolape A F Ilusanya\",\"doi\":\"10.1177/11786302251342936\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>This study investigated the carriage of Plasmid-Mediated Quinolone Resistance (PMQR) genes in fluoroquinolone-resistant <i>Escherichia coli</i> recovered from wastewater generated by healthcare institutions.</p><p><strong>Materials and methods: </strong>Isolation of fluoroquinolone-resistant <i>Escherichia coli</i> was done on medium supplemented with 1 µg/mL of ciprofloxacin (a fluoroquinolone). Presumptive isolates were identified via the detection of <i>uidA</i> gene. Susceptibility of the isolates to a panel of antibiotics was done using disc diffusion method. Detection of PMQR genes in the isolates was done using primer-specific PCR.</p><p><strong>Results: </strong>Thirty fluoroquinolone-resistant <i>Escherichia coli</i> were obtained from the wastewater over a period of 6 months. The resistance to each of the antibiotic tested was: ampicillin (100%), ceftriaxone (100%), nalidixic acid (100%), tetracycline (96.7%), cefotaxime (96.7%), amoxicillin-clavulanate (80%), gentamicin (60%), cefoxitin (30%), and imipenem (3.3%). The Multiple Antibiotic Resistance Index (MARI) ranged from 0.6 to 0.9. The detection of PMQR genes in the 30 isolates was: <i>qnrA</i> (76.7%), <i>qnrB</i> (53.3%), <i>qnrS</i> (63.3%), <i>aac(6')-lb-cr</i> (43.3%), and <i>qepA</i> (43.3%). All the fluoroquinolone-resistant <i>Escherichia coli</i> carried at least one PMQR determinant.</p><p><strong>Conclusion: </strong>This study revealed that untreated hospital wastewaters are significant hub of multidrug-resistant and fluoroquinolone-resistant <i>Escherichia coli</i>, showing high carriage of PMQR genes, and may be a major contributor to the resistome of fluoroquinolone-resistant bacteria in the Nigerian environment.</p>\",\"PeriodicalId\":11827,\"journal\":{\"name\":\"Environmental Health Insights\",\"volume\":\"19 \",\"pages\":\"11786302251342936\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183395/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Health Insights\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/11786302251342936\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Health Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/11786302251342936","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Molecular Characterization of Plasmid-Mediated Quinolone Resistance Genes in Multidrug-Resistant Escherichia coli Isolated From Wastewater Generated From the Hospital Environment.
Aim: This study investigated the carriage of Plasmid-Mediated Quinolone Resistance (PMQR) genes in fluoroquinolone-resistant Escherichia coli recovered from wastewater generated by healthcare institutions.
Materials and methods: Isolation of fluoroquinolone-resistant Escherichia coli was done on medium supplemented with 1 µg/mL of ciprofloxacin (a fluoroquinolone). Presumptive isolates were identified via the detection of uidA gene. Susceptibility of the isolates to a panel of antibiotics was done using disc diffusion method. Detection of PMQR genes in the isolates was done using primer-specific PCR.
Results: Thirty fluoroquinolone-resistant Escherichia coli were obtained from the wastewater over a period of 6 months. The resistance to each of the antibiotic tested was: ampicillin (100%), ceftriaxone (100%), nalidixic acid (100%), tetracycline (96.7%), cefotaxime (96.7%), amoxicillin-clavulanate (80%), gentamicin (60%), cefoxitin (30%), and imipenem (3.3%). The Multiple Antibiotic Resistance Index (MARI) ranged from 0.6 to 0.9. The detection of PMQR genes in the 30 isolates was: qnrA (76.7%), qnrB (53.3%), qnrS (63.3%), aac(6')-lb-cr (43.3%), and qepA (43.3%). All the fluoroquinolone-resistant Escherichia coli carried at least one PMQR determinant.
Conclusion: This study revealed that untreated hospital wastewaters are significant hub of multidrug-resistant and fluoroquinolone-resistant Escherichia coli, showing high carriage of PMQR genes, and may be a major contributor to the resistome of fluoroquinolone-resistant bacteria in the Nigerian environment.