The effect of the cspA gene on growth development and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona

Cold shock proteins (CSPs) represent a universal class of proteins in microorganisms, rapidly inducible under low temperature conditions. As molecular chaperones for RNA, they bind to single-stranded nucleotides, preventing the formation of complex secondary structures. This facilitates efficient translation and gene expression regulation. This investigation pioneers the study of the cspA gene through metabolic engineering techniques, to uncover its critical biological roles in the growth and development of Saccharopolyspora pogona and in butenyl-spinosyn biosynthesis. Employing comparative proteomic and targeted metabolomic analyses, this research elucidates the metabolic pathway alterations prompted by the augmented presence of the cold shock protein CspA. Additionally, it offers initial insights into the regulatory mechanisms by which CspA affects S. pogona's growth, development, and butenyl-spinosyn production. The outcomes of this study significantly advance our theoretical understanding of the rational optimization of butenyl-spinosyn biosynthetic pathways. They also provide valuable guidance for other actinobacteria aiming to boost their resilience to harsh environments by overexpressing the cspA gene.