低甲基化ZNF793在胃癌中的致癌作用：对细胞存活和干细胞的关注

IF 5.1 1区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gastric Cancer Pub Date : 2025-09-01 Epub Date: 2025-06-22 DOI:10.1007/s10120-025-01632-8

Lingyan Jin, Hye-Yeong Jin, Meihui Li, Younghoon Kim, Nam-Yun Cho, Jeong Mo Bae, Gyeong Hoon Kang

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引用次数: 0

摘要

背景：虽然Epstein-Barr病毒相关胃癌（EBV GC）的全基因组启动子CpG岛高甲基化是众所周知的，但ZNF793在EBV GC中很少甲基化，但在其他GC分子亚型中却经常甲基化，包括微卫星不稳定性高的GC。基于ZNF793可能对EBV GC细胞存活和干细胞发育起重要作用的假设，我们研究了ZNF793的致癌作用。方法：在EBV和非EBV GC细胞株中敲除并恢复ZNF793的表达，观察其对细胞增殖、细胞迁移、细胞侵袭、肿瘤球形成和异种移植物肿瘤形成的影响。结果：敲除ZNF793显著抑制了表达ZNF793的GC细胞的迁移、侵袭、增殖和干性。此外，在异种移植肿瘤模型中，敲除ZNF793显著抑制肿瘤生长。结论：这些结果表明ZNF793不仅在EBV GC中起致瘤作用，而且在其他GC亚型中也起作用，可能是一个潜在的治疗靶点。

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The oncogenic role of hypomethylated ZNF793 in gastric carcinoma: a focus on cell survival and stemness.

Background: Although genome-wide promoter CpG island hypermethylation of Epstein-Barr virus-associated gastric carcinoma (EBV GC) is well known, ZNF793 is rarely methylated in EBV GC but is frequently methylated in other molecular subtypes of GC, including microsatellite instability-high GC. Based on the hypothesis that ZNF793 may be important for cell survival and stemness in EBV GC, the oncogenic role of ZNF793 was investigated.

Methods: ZNF793 expression was knocked out and then restored in EBV and non-EBV GC cell lines, and its effects on cell proliferation, cell migration, cell invasion, tumor sphere formation, and xenograft tumor formation were assessed.

Results: ZNF793 knockout significantly suppressed the migration, invasion, proliferation, and stemness in GC cells with ZNF793 expression. Additionally, ZNF793 knockout significantly inhibited tumor growth in a xenograft tumor model.

Conclusions: These results suggest that ZNF793 plays an oncogenic role in not only EBV GC but also other subtypes of GC and may be a potential therapeutic target.

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来源期刊

Gastric Cancer 医学-胃肠肝病学

CiteScore

14.70

自引率

2.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Gastric Cancer is an esteemed global forum that focuses on various aspects of gastric cancer research, treatment, and biology worldwide. The journal promotes a diverse range of content, including original articles, case reports, short communications, and technical notes. It also welcomes Letters to the Editor discussing published articles or sharing viewpoints on gastric cancer topics. Review articles are predominantly sought after by the Editor, ensuring comprehensive coverage of the field. With a dedicated and knowledgeable editorial team, the journal is committed to providing exceptional support and ensuring high levels of author satisfaction. In fact, over 90% of published authors have expressed their intent to publish again in our esteemed journal.