{"title":"RIPOR1表达升高预示结直肠癌患者预后不良。","authors":"Xiaolei Yue, Junyu Bian, Zhikun Dong, Yonglin Chen","doi":"10.21037/tcr-24-2029","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is considered one of the main causes of cancer-related deaths. Rho family-interacting cell polarization regulator 1 (<i>RIPOR1</i>) is a new Rho effector protein, and its abnormal expression may be related to various tumors. However, its expression and significance in CRC are still unclear. We aimed to explore its role and potential value in CRC, and to provide a new theoretical basis of prognostic evaluation and targeted therapy for CRC patients.</p><p><strong>Methods: </strong>Here we investigated the expression and significance of <i>RIPOR1</i> from public databases by using bioinformatics methods. Immunohistochemistry (IHC) was performed to determine protein expression levels of <i>RIPOR1</i> in CRC patients, and the correlation between <i>RIPOR1</i> expression and clinicopathological characteristics was analyzed. <i>In vitro</i> cellular experiments were used to explore the role of <i>RIPOR1</i> in CRC cell lines.</p><p><strong>Results: </strong>Both bioinformatics analysis and IHC results demonstrated that <i>RIPOR1</i> was significantly overexpressed in CRC, which was associated with a worse prognosis. The microRNA (miRNA) database showed that hsa-miR-625-5p was significantly and negatively correlated with <i>RIPOR1</i>. <i>RIPOR1</i> may affect immune cell infiltration in CRC. Cell Counting Kit-8 (CCK-8) assay revealed that <i>RIPOR1</i> promoted cell proliferation in CRC.</p><p><strong>Conclusions: </strong><i>RIPOR1</i> has a significant impact on the development and proliferation of CRC, and may be a potential predictive biomarker.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2707-2721"},"PeriodicalIF":1.5000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170205/pdf/","citationCount":"0","resultStr":"{\"title\":\"Increased expression of <i>RIPOR1</i> predicts the poor prognosis of colorectal cancer patients.\",\"authors\":\"Xiaolei Yue, Junyu Bian, Zhikun Dong, Yonglin Chen\",\"doi\":\"10.21037/tcr-24-2029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Colorectal cancer (CRC) is considered one of the main causes of cancer-related deaths. Rho family-interacting cell polarization regulator 1 (<i>RIPOR1</i>) is a new Rho effector protein, and its abnormal expression may be related to various tumors. However, its expression and significance in CRC are still unclear. We aimed to explore its role and potential value in CRC, and to provide a new theoretical basis of prognostic evaluation and targeted therapy for CRC patients.</p><p><strong>Methods: </strong>Here we investigated the expression and significance of <i>RIPOR1</i> from public databases by using bioinformatics methods. Immunohistochemistry (IHC) was performed to determine protein expression levels of <i>RIPOR1</i> in CRC patients, and the correlation between <i>RIPOR1</i> expression and clinicopathological characteristics was analyzed. <i>In vitro</i> cellular experiments were used to explore the role of <i>RIPOR1</i> in CRC cell lines.</p><p><strong>Results: </strong>Both bioinformatics analysis and IHC results demonstrated that <i>RIPOR1</i> was significantly overexpressed in CRC, which was associated with a worse prognosis. The microRNA (miRNA) database showed that hsa-miR-625-5p was significantly and negatively correlated with <i>RIPOR1</i>. <i>RIPOR1</i> may affect immune cell infiltration in CRC. Cell Counting Kit-8 (CCK-8) assay revealed that <i>RIPOR1</i> promoted cell proliferation in CRC.</p><p><strong>Conclusions: </strong><i>RIPOR1</i> has a significant impact on the development and proliferation of CRC, and may be a potential predictive biomarker.</p>\",\"PeriodicalId\":23216,\"journal\":{\"name\":\"Translational cancer research\",\"volume\":\"14 5\",\"pages\":\"2707-2721\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170205/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Translational cancer research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21037/tcr-24-2029\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/12 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Translational cancer research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21037/tcr-24-2029","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/12 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
Increased expression of RIPOR1 predicts the poor prognosis of colorectal cancer patients.
Background: Colorectal cancer (CRC) is considered one of the main causes of cancer-related deaths. Rho family-interacting cell polarization regulator 1 (RIPOR1) is a new Rho effector protein, and its abnormal expression may be related to various tumors. However, its expression and significance in CRC are still unclear. We aimed to explore its role and potential value in CRC, and to provide a new theoretical basis of prognostic evaluation and targeted therapy for CRC patients.
Methods: Here we investigated the expression and significance of RIPOR1 from public databases by using bioinformatics methods. Immunohistochemistry (IHC) was performed to determine protein expression levels of RIPOR1 in CRC patients, and the correlation between RIPOR1 expression and clinicopathological characteristics was analyzed. In vitro cellular experiments were used to explore the role of RIPOR1 in CRC cell lines.
Results: Both bioinformatics analysis and IHC results demonstrated that RIPOR1 was significantly overexpressed in CRC, which was associated with a worse prognosis. The microRNA (miRNA) database showed that hsa-miR-625-5p was significantly and negatively correlated with RIPOR1. RIPOR1 may affect immune cell infiltration in CRC. Cell Counting Kit-8 (CCK-8) assay revealed that RIPOR1 promoted cell proliferation in CRC.
Conclusions: RIPOR1 has a significant impact on the development and proliferation of CRC, and may be a potential predictive biomarker.
期刊介绍：
Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.
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