Chunyan Xu, Yingbin Hu, Guodong Dai, Yan Chen, Chengxia Liu
{"title":"SphK1抑制剂SKI ii -培养基中分离的细胞外囊泡抑制结直肠癌的迁移。","authors":"Chunyan Xu, Yingbin Hu, Guodong Dai, Yan Chen, Chengxia Liu","doi":"10.21037/tcr-24-2152","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer is a chronic disease particularly detrimental to human health. Although there have been many studies on colorectal cancer and extracellular vesicles (EVs), it is unknown whether SKI II, an inhibitor of sphingosine kinase 1 (SphK1), uses EVs as transporters to inhibit colorectal cancer migration. This research aimed to investigate whether EVs, which were isolated from SphK1 inhibitor SKI II-medium, affected on E-cadherin and vimentin and the cell migration of colorectal cancer cells.</p><p><strong>Methods: </strong>EVs were extracted from RKO cells using an exosome extraction and purification kit, and the extracted EVs were identified to evaluate whether the EVs extracted by this kit met the experimental requirements. <sup>RKO</sup>EVs were extracted from RKO exosom-free serum culture medium, and <sup>RKO-SK</sup>EVs were extracted from RKO exosom-free serum culture medium with SKI II intervention. PKH67-labeled EVs were added to the cells. EVs inhibitor GW4869, <sup>RKO</sup>EVs, and <sup>RKO-SK</sup>EVs were used to intervene in RKO cells, and <sup>RKO</sup>EVs and <sup>RKO-SK</sup>EVs were used to intervene in HT29 cells. The E-cadherin and vimentin expression were tested by western blotting. Transwell assay was used to detect cell migration ability.</p><p><strong>Results: </strong>Compared with the control group, after GW4869 treatment, E-cadherin was increased, vimentin was decreased, and the number of migrating cells was decreased. After <sup>RKO</sup>EVs intervention, the expression of E-cadherin, vimentin and cell migration number were opposite. Compared with <sup>RKO</sup>EVs intervention in RKO cells, E-cadherin was increased, vimentin was decreased and the number of migrating cells decreased after <sup>RKO-SK</sup>EVs intervention. Similarly, compared with <sup>RKO</sup>EVs intervention in HT29 cells, after <sup>RKO-SK</sup>EVs intervention, E-cadherin was increased, vimentin was decreased, and the number of migrating cells decreased.</p><p><strong>Conclusions: </strong>EVs isolated from SphK1 inhibitor SKI II-medium affect E-cadherin and vimentin expression in colorectal cancer and inhibit cell migration.</p>","PeriodicalId":23216,"journal":{"name":"Translational cancer research","volume":"14 5","pages":"2594-2602"},"PeriodicalIF":1.5000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170209/pdf/","citationCount":"0","resultStr":"{\"title\":\"Extracellular vesicles isolated from SphK1 inhibitor SKI II-medium restrain the migration of colorectal cancer.\",\"authors\":\"Chunyan Xu, Yingbin Hu, Guodong Dai, Yan Chen, Chengxia Liu\",\"doi\":\"10.21037/tcr-24-2152\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Colorectal cancer is a chronic disease particularly detrimental to human health. Although there have been many studies on colorectal cancer and extracellular vesicles (EVs), it is unknown whether SKI II, an inhibitor of sphingosine kinase 1 (SphK1), uses EVs as transporters to inhibit colorectal cancer migration. This research aimed to investigate whether EVs, which were isolated from SphK1 inhibitor SKI II-medium, affected on E-cadherin and vimentin and the cell migration of colorectal cancer cells.</p><p><strong>Methods: </strong>EVs were extracted from RKO cells using an exosome extraction and purification kit, and the extracted EVs were identified to evaluate whether the EVs extracted by this kit met the experimental requirements. <sup>RKO</sup>EVs were extracted from RKO exosom-free serum culture medium, and <sup>RKO-SK</sup>EVs were extracted from RKO exosom-free serum culture medium with SKI II intervention. PKH67-labeled EVs were added to the cells. EVs inhibitor GW4869, <sup>RKO</sup>EVs, and <sup>RKO-SK</sup>EVs were used to intervene in RKO cells, and <sup>RKO</sup>EVs and <sup>RKO-SK</sup>EVs were used to intervene in HT29 cells. The E-cadherin and vimentin expression were tested by western blotting. Transwell assay was used to detect cell migration ability.</p><p><strong>Results: </strong>Compared with the control group, after GW4869 treatment, E-cadherin was increased, vimentin was decreased, and the number of migrating cells was decreased. After <sup>RKO</sup>EVs intervention, the expression of E-cadherin, vimentin and cell migration number were opposite. Compared with <sup>RKO</sup>EVs intervention in RKO cells, E-cadherin was increased, vimentin was decreased and the number of migrating cells decreased after <sup>RKO-SK</sup>EVs intervention. Similarly, compared with <sup>RKO</sup>EVs intervention in HT29 cells, after <sup>RKO-SK</sup>EVs intervention, E-cadherin was increased, vimentin was decreased, and the number of migrating cells decreased.</p><p><strong>Conclusions: </strong>EVs isolated from SphK1 inhibitor SKI II-medium affect E-cadherin and vimentin expression in colorectal cancer and inhibit cell migration.</p>\",\"PeriodicalId\":23216,\"journal\":{\"name\":\"Translational cancer research\",\"volume\":\"14 5\",\"pages\":\"2594-2602\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170209/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Translational cancer research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21037/tcr-24-2152\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Translational cancer research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21037/tcr-24-2152","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/7 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
Extracellular vesicles isolated from SphK1 inhibitor SKI II-medium restrain the migration of colorectal cancer.
Background: Colorectal cancer is a chronic disease particularly detrimental to human health. Although there have been many studies on colorectal cancer and extracellular vesicles (EVs), it is unknown whether SKI II, an inhibitor of sphingosine kinase 1 (SphK1), uses EVs as transporters to inhibit colorectal cancer migration. This research aimed to investigate whether EVs, which were isolated from SphK1 inhibitor SKI II-medium, affected on E-cadherin and vimentin and the cell migration of colorectal cancer cells.
Methods: EVs were extracted from RKO cells using an exosome extraction and purification kit, and the extracted EVs were identified to evaluate whether the EVs extracted by this kit met the experimental requirements. RKOEVs were extracted from RKO exosom-free serum culture medium, and RKO-SKEVs were extracted from RKO exosom-free serum culture medium with SKI II intervention. PKH67-labeled EVs were added to the cells. EVs inhibitor GW4869, RKOEVs, and RKO-SKEVs were used to intervene in RKO cells, and RKOEVs and RKO-SKEVs were used to intervene in HT29 cells. The E-cadherin and vimentin expression were tested by western blotting. Transwell assay was used to detect cell migration ability.
Results: Compared with the control group, after GW4869 treatment, E-cadherin was increased, vimentin was decreased, and the number of migrating cells was decreased. After RKOEVs intervention, the expression of E-cadherin, vimentin and cell migration number were opposite. Compared with RKOEVs intervention in RKO cells, E-cadherin was increased, vimentin was decreased and the number of migrating cells decreased after RKO-SKEVs intervention. Similarly, compared with RKOEVs intervention in HT29 cells, after RKO-SKEVs intervention, E-cadherin was increased, vimentin was decreased, and the number of migrating cells decreased.
Conclusions: EVs isolated from SphK1 inhibitor SKI II-medium affect E-cadherin and vimentin expression in colorectal cancer and inhibit cell migration.
期刊介绍：
Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.
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