生物发酵半聚脲前体肽在大孔树脂上吸附过程的研究

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2025-06-17 DOI:10.1016/j.fbp.2025.06.006

Zhewei Zhang, Ruopeng Li, Lin Zhang, Zhengfei Ma, Haiyan Wang, Qun Cui

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引用次数: 0

摘要

Semaglutide是第二代胰高血糖素样肽-1受体激动剂（GLP-1RA），已被公认为2型糖尿病治疗的一线药物。semaglutide前体肽（SPP）的纯化是semaglutide产品生物制药生产过程中至关重要的质量决定步骤。对三种大孔树脂（D3520、XAD7HP和BMKX-4）进行了筛选和表征。分析了SPP在XAD7HP上的吸附热力学和动力学特性。考察了SPP的吸附分离条件和XAD7HP的吸附/解吸稳定性。结果表明：XAD7HP的强极性碳氧双键能与SPP的氨基形成氢键，SPP在XAD7HP上的吸附过程符合Langmuir模型和拟二级动力学模型。在25 ℃下，SPP对XAD7HP的最大吸附量为164.21 mg/g，回收率为75 %。在60 %乙醇（v/v）条件下，SPP对XAD7HP的解吸效率为96.64 %，XAD7HP在15次吸附-解吸循环中表现出良好的稳定性。这项工作为生物多肽的纯化提供了一种可扩展和稳定的方法，克服了传统非特异性吸附方法的局限性。

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An investigation into adsorption process of biologically- fermented semaglutide precursor peptide on macroporous resins

Semaglutide, a second-generation glucagon-like peptide-1 receptor agonist (GLP-1RA), has been recognized as a first-line therapeutic agent in type 2 diabetes management. The purification of semaglutide precursor peptide (SPP) constitutes a crucial quality-determining step in the biopharmaceutical manufacturing process of semaglutide products. Three macroporous resins (D3520, XAD7HP, and BMKX-4) were screened and characterized. The adsorption thermodynamic and kinetic characteristics of SPP on XAD7HP were analyzed. Adsorption separation conditions of SPP and adsorption/desorption stability of XAD7HP were evaluated. The results indicated that the strong polar carbon-oxygen double bonds in XAD7HP could form hydrogen bonds with the amino groups of SPP. The adsorption process of SPP on XAD7HP was consistent with the Langmuir model and the pseudo-second-order kinetic model. At 25 ℃, the maximum adsorption capacity of SPP on XAD7HP reached 164.21 mg/g, and the recovery rate was 75 %. Desorption efficiency of SPP on XAD7HP was 96.64 % using a 60 % ethanol (v/v) solution, and XAD7HP exhibited excellent stability within 15 adsorption-desorption cycles. This work offered a scalable and stable purification approach for biological peptides that circumvents limitations of traditional non-specific adsorption methods.

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.