Jeric Mun Chung Kwan, Shiliu Feng, Evan Wei Long Ng and Yuan Qiao
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Biochemical characterization of Bifidobacterium bifidum peptidoglycan d,l-endopeptidase BbMep that generates NOD2 ligands†
Soluble peptidoglycan fragments produced by the gut bacteria are key effectors in microbiota–host crosstalk. Here, we biochemically characterized BbMep, an NlpC/p60 domain-containing peptidoglycan D,L-endopeptidase from Bifidobacterium bifidum, which efficiently digests Lys- or Orn-type sacculi. Digestion of human stool-derived muropeptides by BbMep enhances NOD2 activation.
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