静止骨骼肌的性别差异以及超重和肥胖个体对耐力运动的急性和长期反应。

IF 6.6 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Molecular Metabolism Pub Date : 2025-06-16 DOI:10.1016/j.molmet.2025.102185

Simon I. Dreher , Thomas Goj , Christine von Toerne , Miriam Hoene , Martin Irmler , Meriem Ouni , Markus Jähnert , Johannes Beckers , Martin Hrabě de Angelis , Andreas Peter , Anja Moller , Andreas L. Birkenfeld , Annette Schürmann , Stefanie M. Hauck , Cora Weigert

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引用次数: 0

摘要

耐力运动通过改善骨骼肌代谢，尤其是超重和肥胖的人，降低了患代谢性疾病的风险。由于生理性别影响骨骼肌中葡萄糖和脂肪酸的处理，我们假设在运动和8周运动干预后的急性反应中转录组学和蛋白质组学水平上的性别差异。我们使用转录组学和蛋白质组学分析了25名超重和肥胖久坐受试者（16f/9m）在基线、急性运动后和8周耐力训练计划后的骨骼肌活检。研究了供体原代肌管中性别特异性差异的调节。在基线上，差异甲基化的cpg位点可能解释高达59%的转录组性差异和67%的蛋白质组性差异。差异主要表现在雄性中快速收缩纤维型蛋白的丰度较高，以及调节糖原降解和糖酵解的转录本和蛋白。雌性显示出更高丰度的调节脂肪酸摄取和储存的蛋白质。急性运动诱导应激反应转录物和血清肌红蛋白主要在男性。通过上调参与TCA循环、氧化磷酸化和β-氧化的线粒体蛋白，男女都适应了8周的耐力训练。训练平衡了快肌纤维类型的蛋白质水平，主要是通过降低男性的水平。体内常染色体基因的性别差异在肌管中保留得很差，但通过性激素治疗部分恢复。总之，我们的研究结果突出了性别特异性分子特征，反映了女性和男性骨骼肌之间葡萄糖和脂质代谢的已知差异。仅仅经过8周的耐力训练，这些性别差异就减弱了，这表明在分子水平上趋同于共同的有益适应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity

查看原文本刊更多论文

Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity

Objectives

Endurance exercise reduces the risk of metabolic diseases by improving skeletal muscle metabolism, particularly in individuals with overweight and obesity. As biological sex impacts glucose and fatty acid handling in skeletal muscle, we hypothesized sex differences at the transcriptomic and proteomic level in the acute response to exercise and after an 8-week exercise intervention.

Methods

We analyzed skeletal muscle biopsies from 25 sedentary subjects (16f/9 m) with overweight and obesity using transcriptomics and proteomics at baseline, after acute exercise, and following an 8-week endurance training program. Regulation of sex-specific differences was studied in primary myotubes from the donors.

Results

At baseline, differentially methylated CpG-sites potentially explain up to 59% of transcriptomic and 67% of proteomic sex differences. Differences were dominated by higher abundance of fast-twitch fiber type proteins, and transcripts and proteins regulating glycogen degradation and glycolysis in males. Females showed higher abundance of proteins regulating fatty acid uptake and storage. Acute exercise induced stress-responsive transcripts and serum myoglobin predominantly in males. Both sexes adapted to 8-week endurance training by upregulating mitochondrial proteins involved in TCA cycle, oxidative phosphorylation, and β-oxidation. Training equalized fast-twitch fiber type protein levels, mainly by reducing them in males. In vivo sex differences in autosomal genes were poorly retained in myotubes but partially restored by sex hormone treatment.

Conclusions

Our findings highlight sex-specific molecular signatures that reflect known differences in glucose and lipid metabolism between female and male skeletal muscle. After just 8 weeks of endurance training, these sex differences were attenuated, suggesting a convergence towards a shared beneficial adaptation at the molecular level.

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来源期刊

Molecular Metabolism ENDOCRINOLOGY & METABOLISM-

CiteScore

14.50

自引率

2.50%

发文量

219

审稿时长

43 days

期刊介绍： Molecular Metabolism is a leading journal dedicated to sharing groundbreaking discoveries in the field of energy homeostasis and the underlying factors of metabolic disorders. These disorders include obesity, diabetes, cardiovascular disease, and cancer. Our journal focuses on publishing research driven by hypotheses and conducted to the highest standards, aiming to provide a mechanistic understanding of energy homeostasis-related behavior, physiology, and dysfunction. We promote interdisciplinary science, covering a broad range of approaches from molecules to humans throughout the lifespan. Our goal is to contribute to transformative research in metabolism, which has the potential to revolutionize the field. By enabling progress in the prognosis, prevention, and ultimately the cure of metabolic disorders and their long-term complications, our journal seeks to better the future of health and well-being.