木质素降解微生物和木质素氧化酶的测定方法和比色筛。

4区生物学 Q3 Biochemistry, Genetics and Molecular Biology

Methods in enzymology Pub Date : 2025-01-01 Epub Date: 2025-02-16 DOI:10.1016/bs.mie.2025.01.055

Timothy D H Bugg, Mark Ahmad, Charles R Taylor, Marina Konstantopoulou, Goran M M Rashid

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引用次数: 0

摘要

测定酶和微生物降解聚合木质素的活性本身就是具有挑战性的。本文介绍了本课题组开发的几种测定木质素氧化酶和木质素降解微生物的方法。测定方法包括：(1)化学硝化木质素的比色测定；(2)凝胶过滤色谱法测定分子量变化；(3)克拉森法木质纤维素脱木质素；(4)低分子量酚类和羰基化合物释放比色法测定。

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Assay methods and colorimetric screens for lignin-degrading microbes and lignin-oxidising enzymes.

Assaying enzymes and microbes for activity for degradation of polymeric lignin is inherently challenging to do. This article describes several methods that our research group has developed for assay of lignin-oxidising enzymes and lignin-degrading microbes. The assay methods involve (1) colorimetric assays involving chemically nitrated lignin; (2) changes in molecular weight using gel filtration chromatography; (3) delignification of lignocellulose using Klason assay; (4) colorimetric assays for release of low molecular weight phenols and carbonyl compounds.

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来源期刊

Methods in enzymology 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

308

审稿时长

3-6 weeks

期刊介绍： The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.