寡雄激素通过水杨酸和茉莉酸信号通路增强樱桃番茄收获后抗病性的作用

IF 3.7 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Applied and Environmental Microbiology Pub Date : 2025-07-23 Epub Date: 2025-06-13 DOI:10.1128/aem.00421-25
Jia-Hao Sun, Jia-Hui Xu, Chuanzhi Kang, Lu Cheng, Yu-Tang Gao, Feng-Qiao Hu, Jian Liang, Lan-Ping Guo, Xiao-Kui Ma
{"title":"寡雄激素通过水杨酸和茉莉酸信号通路增强樱桃番茄收获后抗病性的作用","authors":"Jia-Hao Sun, Jia-Hui Xu, Chuanzhi Kang, Lu Cheng, Yu-Tang Gao, Feng-Qiao Hu, Jian Liang, Lan-Ping Guo, Xiao-Kui Ma","doi":"10.1128/aem.00421-25","DOIUrl":null,"url":null,"abstract":"<p><p>Salicylic acid (SA) and jasmonic acid (JA) pathways are crucial components of plant defense mechanisms; however, their roles in post-harvest preservation remain inadequately explored. Cherry tomatoes are highly susceptible to fungal pathogens, such as <i>Alternaria nees</i>, after harvest, leading to significant post-harvest losses. This study investigates the effects of oligandrin, an elicitin protein derived from <i>Pythium oligandrum</i>, on enhancing the resistance of harvested cherry tomatoes to <i>A. nees</i>. Oligandrin treatment significantly reduced disease symptoms, including black spots and decay, during storage without affecting <i>A. nees</i> spore germination. Treated samples exhibited enhanced activities of defense-related enzymes, including polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and catalase (CAT), compared to controls. Key SA-responsive genes, such as <i>NPR1</i> (Non-expressor of Pathogenesis-Related Genes 1), <i>TGA2</i> (TGACG-Binding Factor 2), <i>WRKY70</i>, and <i>PR1</i> (Pathogenesis-Related Protein 1), were upregulated within 48 h, indicating activation of the SA signaling pathway. Additionally, <i>JAZ1</i> (Jasmonate ZIM-Domain 1), a negative regulator of JA signaling, was elevated at both 24 and 48 h, while <i>MYC2</i> (Myelocytomatosis 2), a transcription factor involved in the JA pathway, showed significant upregulation, suggesting activation of the JA signaling pathway. These findings demonstrate that oligandrin enhances post-harvest fungal disease resistance by modulating both SA and JA pathways and sustaining elevated defense enzyme activity, offering a promising strategy for improving post-harvest preservation and quality maintenance of cherry tomatoes.IMPORTANCEThis study provides compelling evidence that oligandrin activates both salicylic acid and jasmonic acid signaling pathways in cherry tomatoes, enhancing resistance to <i>A. nees</i>. This dual activation not only deepens our understanding of oligandrin's mechanisms but also underscores the potential of oligandrin and its producer <i>P. oligandrum</i> as a biocontrol agent for post-harvest preservation. By modulating plant immunity and promoting defense enzyme activity, oligandrin strengthens resistance to fungal diseases, offering a promising strategy for maintaining the quality and extending the shelf life of berry-like crops. Given the successful use of <i>P. oligandrum</i> as a biopesticide in Europe and North America, oligandrin and its producer <i>P. oligandrum</i> present an environmentally friendly alternative to chemical pesticides for post-harvest disease management. This research lays the groundwork for further studies aimed at optimizing the application of <i>P. oligandrum</i> in real-world agricultural settings, establishing it as a reliable, sustainable tool for both agricultural and post-harvest management practices. These findings contribute to advancing biocontrol strategies for safer, more sustainable food production systems.</p>","PeriodicalId":8002,"journal":{"name":"Applied and Environmental Microbiology","volume":" ","pages":"e0042125"},"PeriodicalIF":3.7000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285221/pdf/","citationCount":"0","resultStr":"{\"title\":\"Role of oligandrin in enhancing post-harvest disease resistance in cherry tomato through salicylic acid and jasmonic acid signaling pathways.\",\"authors\":\"Jia-Hao Sun, Jia-Hui Xu, Chuanzhi Kang, Lu Cheng, Yu-Tang Gao, Feng-Qiao Hu, Jian Liang, Lan-Ping Guo, Xiao-Kui Ma\",\"doi\":\"10.1128/aem.00421-25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Salicylic acid (SA) and jasmonic acid (JA) pathways are crucial components of plant defense mechanisms; however, their roles in post-harvest preservation remain inadequately explored. Cherry tomatoes are highly susceptible to fungal pathogens, such as <i>Alternaria nees</i>, after harvest, leading to significant post-harvest losses. This study investigates the effects of oligandrin, an elicitin protein derived from <i>Pythium oligandrum</i>, on enhancing the resistance of harvested cherry tomatoes to <i>A. nees</i>. Oligandrin treatment significantly reduced disease symptoms, including black spots and decay, during storage without affecting <i>A. nees</i> spore germination. Treated samples exhibited enhanced activities of defense-related enzymes, including polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and catalase (CAT), compared to controls. Key SA-responsive genes, such as <i>NPR1</i> (Non-expressor of Pathogenesis-Related Genes 1), <i>TGA2</i> (TGACG-Binding Factor 2), <i>WRKY70</i>, and <i>PR1</i> (Pathogenesis-Related Protein 1), were upregulated within 48 h, indicating activation of the SA signaling pathway. Additionally, <i>JAZ1</i> (Jasmonate ZIM-Domain 1), a negative regulator of JA signaling, was elevated at both 24 and 48 h, while <i>MYC2</i> (Myelocytomatosis 2), a transcription factor involved in the JA pathway, showed significant upregulation, suggesting activation of the JA signaling pathway. These findings demonstrate that oligandrin enhances post-harvest fungal disease resistance by modulating both SA and JA pathways and sustaining elevated defense enzyme activity, offering a promising strategy for improving post-harvest preservation and quality maintenance of cherry tomatoes.IMPORTANCEThis study provides compelling evidence that oligandrin activates both salicylic acid and jasmonic acid signaling pathways in cherry tomatoes, enhancing resistance to <i>A. nees</i>. This dual activation not only deepens our understanding of oligandrin's mechanisms but also underscores the potential of oligandrin and its producer <i>P. oligandrum</i> as a biocontrol agent for post-harvest preservation. By modulating plant immunity and promoting defense enzyme activity, oligandrin strengthens resistance to fungal diseases, offering a promising strategy for maintaining the quality and extending the shelf life of berry-like crops. Given the successful use of <i>P. oligandrum</i> as a biopesticide in Europe and North America, oligandrin and its producer <i>P. oligandrum</i> present an environmentally friendly alternative to chemical pesticides for post-harvest disease management. This research lays the groundwork for further studies aimed at optimizing the application of <i>P. oligandrum</i> in real-world agricultural settings, establishing it as a reliable, sustainable tool for both agricultural and post-harvest management practices. These findings contribute to advancing biocontrol strategies for safer, more sustainable food production systems.</p>\",\"PeriodicalId\":8002,\"journal\":{\"name\":\"Applied and Environmental Microbiology\",\"volume\":\" \",\"pages\":\"e0042125\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285221/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied and Environmental Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1128/aem.00421-25\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/13 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied and Environmental Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1128/aem.00421-25","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/13 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

水杨酸(SA)和茉莉酸(JA)途径是植物防御机制的重要组成部分;然而,它们在收获后保存中的作用仍未得到充分探讨。圣女果在收获后极易受到真菌病原体的影响,如赤霉病,导致收获后的重大损失。本文研究了寡雄激素(一种从寡雄激素中提取的卵磷脂蛋白)对提高收获的圣女果对A. nees的抗性的作用。寡雄素处理显著减少了贮藏期间的疾病症状,包括黑斑和腐烂,而不影响白藜芦醇孢子的萌发。与对照组相比,处理过的样品显示出防御相关酶的活性增强,包括多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)和过氧化氢酶(CAT)。关键SA应答基因,如NPR1(发病相关基因1非表达者)、TGA2 (tgacg结合因子2)、WRKY70和PR1(发病相关蛋白1)在48 h内上调,表明SA信号通路被激活。此外,JA信号的负调节因子JAZ1 (Jasmonate ZIM-Domain 1)在24和48 h时均升高,而参与JA信号通路的转录因子MYC2 (Myelocytomatosis 2)显著上调,提示JA信号通路被激活。这些研究结果表明,寡雄激素通过调节SA和JA途径以及维持防御酶活性升高来增强收获后的真菌抗病能力,为改善樱桃番茄收获后的保存和品质维持提供了一种有希望的策略。这项研究提供了令人信服的证据,证明寡雄激素激活了圣女果的水杨酸和茉莉酸信号通路,增强了对A. nees的抗性。这种双重激活不仅加深了我们对寡雄激素机制的理解,而且强调了寡雄激素及其生产者寡雄激素作为收获后保存生物防治剂的潜力。寡雄素通过调节植物免疫和促进防御酶活性,增强对真菌病害的抗性,为保持浆果类作物的品质和延长保质期提供了一种有前途的策略。鉴于在欧洲和北美,寡雄豆作为生物农药的成功使用,寡雄豆素及其生产者寡雄豆为收获后病害管理提供了一种环境友好的化学农药替代品。该研究为进一步研究奠定了基础,旨在优化在现实农业环境中的应用,使其成为农业和收获后管理实践的可靠、可持续的工具。这些发现有助于推进生物防治战略,以实现更安全、更可持续的粮食生产系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of oligandrin in enhancing post-harvest disease resistance in cherry tomato through salicylic acid and jasmonic acid signaling pathways.

Salicylic acid (SA) and jasmonic acid (JA) pathways are crucial components of plant defense mechanisms; however, their roles in post-harvest preservation remain inadequately explored. Cherry tomatoes are highly susceptible to fungal pathogens, such as Alternaria nees, after harvest, leading to significant post-harvest losses. This study investigates the effects of oligandrin, an elicitin protein derived from Pythium oligandrum, on enhancing the resistance of harvested cherry tomatoes to A. nees. Oligandrin treatment significantly reduced disease symptoms, including black spots and decay, during storage without affecting A. nees spore germination. Treated samples exhibited enhanced activities of defense-related enzymes, including polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and catalase (CAT), compared to controls. Key SA-responsive genes, such as NPR1 (Non-expressor of Pathogenesis-Related Genes 1), TGA2 (TGACG-Binding Factor 2), WRKY70, and PR1 (Pathogenesis-Related Protein 1), were upregulated within 48 h, indicating activation of the SA signaling pathway. Additionally, JAZ1 (Jasmonate ZIM-Domain 1), a negative regulator of JA signaling, was elevated at both 24 and 48 h, while MYC2 (Myelocytomatosis 2), a transcription factor involved in the JA pathway, showed significant upregulation, suggesting activation of the JA signaling pathway. These findings demonstrate that oligandrin enhances post-harvest fungal disease resistance by modulating both SA and JA pathways and sustaining elevated defense enzyme activity, offering a promising strategy for improving post-harvest preservation and quality maintenance of cherry tomatoes.IMPORTANCEThis study provides compelling evidence that oligandrin activates both salicylic acid and jasmonic acid signaling pathways in cherry tomatoes, enhancing resistance to A. nees. This dual activation not only deepens our understanding of oligandrin's mechanisms but also underscores the potential of oligandrin and its producer P. oligandrum as a biocontrol agent for post-harvest preservation. By modulating plant immunity and promoting defense enzyme activity, oligandrin strengthens resistance to fungal diseases, offering a promising strategy for maintaining the quality and extending the shelf life of berry-like crops. Given the successful use of P. oligandrum as a biopesticide in Europe and North America, oligandrin and its producer P. oligandrum present an environmentally friendly alternative to chemical pesticides for post-harvest disease management. This research lays the groundwork for further studies aimed at optimizing the application of P. oligandrum in real-world agricultural settings, establishing it as a reliable, sustainable tool for both agricultural and post-harvest management practices. These findings contribute to advancing biocontrol strategies for safer, more sustainable food production systems.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Applied and Environmental Microbiology
Applied and Environmental Microbiology 生物-生物工程与应用微生物
CiteScore
7.70
自引率
2.30%
发文量
730
审稿时长
1.9 months
期刊介绍: Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信