一种新型增强剂促进水牛肌肉发育的机制探讨

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-06-11 DOI:10.1016/j.ygeno.2025.111071

Saixing Duan , Xingyu Liu , Wenjing Liu , Yuting Dou , Gaoxiao Xu , Feng Zhang , Kuan Geng , Qi Xiong , Mengjie Chen

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引用次数: 0

摘要

骨骼肌的发育过程涉及多种调控因素。然而，关于增强剂在水牛骨骼肌生长发育中的作用和机制的报道很少。在这项研究中，我们发现了一种影响水牛骨骼肌生长和发育的增强子，我们将其命名为Enh67。CCK-8、EdU、qPCR和Western blot实验结果表明，Enh67能抑制水牛肌干细胞的增殖。免疫荧光染色、qPCR和Western blot结果显示，Enh67促进了水牛肌干细胞的分化。最后，我们通过3C和qPCR检测确定了Enh67靶向CEP85L。我们的发现不仅丰富了水牛骨骼肌生长发育的调控因子，而且为提高肉类产量和治疗牲畜肌肉发育相关疾病提供了潜在的靶点候选物。

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Exploration of the mechanism of a novel enhancer on buffalo muscle development

The developmental process of skeletal muscle involves a wide variety of regulatory factors. However, little has been reported regarding the function and mechanisms of enhancer regulation in buffalo skeletal muscle growth and development. In this study, we discovered an enhancer that affects the buffalo skeletal muscle growth and development, which we named Enh67. Experimental results from CCK-8, EdU, qPCR, and Western blot assays indicated that Enh67 inhibits the proliferation of buffalo muscle stem cells. Immunofluorescence staining, qPCR, and Western blot results showed that Enh67 promotes the differentiation of buffalo muscle stem cells. Finally, we determined through 3C and qPCR assays that Enh67 targets CEP85L. Our findings not only enrich the regulatory factors involved in buffalo skeletal muscle growth and development but also provide potential target candidates for improving meat production and treating muscle development-related diseases in livestock.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.