{"title":"植物提取物发酵饮料多糖透明质酸酶抑制活性研究。","authors":"Hideki Okada, Akira Yamamori, Naoki Kawazoe, Keiji Ueno, Shuichi Onodera","doi":"10.5458/jag.7202202","DOIUrl":null,"url":null,"abstract":"<p><p>Super Ohtaka<sup>®</sup>, a fermented beverage of plant extracts, is prepared from approximately 50 kinds of vegetables and fruits is a naturally fermented mainly by lactic acid bacteria (<i>Leuconostoc</i> spp.) and yeast (<i>Zygosaccharomyces</i> spp.). In this study, we separated water-soluble polysaccharides from Super Ohtaka<sup>®</sup> using dialysis and chromatography, yielding four polysaccharide fractions. The polysaccharide fraction designated as OEP3 exhibited hyaluronidase inhibitory activity. The half-maximal inhibitory concentration was 860 µg/mL. This polysaccharide not only stimulated macrophages but also inhibited hyaluronidase activity and showed weak 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"72 2","pages":"7202202"},"PeriodicalIF":1.4000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149733/pdf/","citationCount":"0","resultStr":"{\"title\":\"Hyaluronidase Inhibitory Activity of Polysaccharides Separated from a Fermented Beverage of Plant Extracts.\",\"authors\":\"Hideki Okada, Akira Yamamori, Naoki Kawazoe, Keiji Ueno, Shuichi Onodera\",\"doi\":\"10.5458/jag.7202202\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Super Ohtaka<sup>®</sup>, a fermented beverage of plant extracts, is prepared from approximately 50 kinds of vegetables and fruits is a naturally fermented mainly by lactic acid bacteria (<i>Leuconostoc</i> spp.) and yeast (<i>Zygosaccharomyces</i> spp.). In this study, we separated water-soluble polysaccharides from Super Ohtaka<sup>®</sup> using dialysis and chromatography, yielding four polysaccharide fractions. The polysaccharide fraction designated as OEP3 exhibited hyaluronidase inhibitory activity. The half-maximal inhibitory concentration was 860 µg/mL. This polysaccharide not only stimulated macrophages but also inhibited hyaluronidase activity and showed weak 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity.</p>\",\"PeriodicalId\":14999,\"journal\":{\"name\":\"Journal of applied glycoscience\",\"volume\":\"72 2\",\"pages\":\"7202202\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2025-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149733/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of applied glycoscience\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5458/jag.7202202\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied glycoscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5458/jag.7202202","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
Super Ohtaka®是一种植物提取物发酵饮料，由大约50种蔬菜和水果制成，主要由乳酸菌（Leuconostoc spp.）和酵母（Zygosaccharomyces spp.）自然发酵而成。在本研究中，我们用透析和色谱分离了Super Ohtaka®的水溶性多糖，得到了四个多糖部分。多糖部分命名为OEP3，显示出透明质酸酶抑制活性。半最大抑制浓度为860µg/mL。该多糖不仅能刺激巨噬细胞，还能抑制透明质酸酶活性，并表现出较弱的1,1-二苯基-2-苦酰肼自由基清除活性。
Hyaluronidase Inhibitory Activity of Polysaccharides Separated from a Fermented Beverage of Plant Extracts.
Super Ohtaka®, a fermented beverage of plant extracts, is prepared from approximately 50 kinds of vegetables and fruits is a naturally fermented mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp.). In this study, we separated water-soluble polysaccharides from Super Ohtaka® using dialysis and chromatography, yielding four polysaccharide fractions. The polysaccharide fraction designated as OEP3 exhibited hyaluronidase inhibitory activity. The half-maximal inhibitory concentration was 860 µg/mL. This polysaccharide not only stimulated macrophages but also inhibited hyaluronidase activity and showed weak 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity.
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