Superresolution Microscopy Reveals Alterations in Clathrin Structure in Human Cancer Tissue.

Superresolution microscopy holds great promise for detailed structural analysis of proteins, yet its application in the investigations of protein structures in situ remains sparse. Clathrin-coated pit-mediated endocytosis (CME) plays a key role in human cancer. This study aimed to discover whether there are structural changes in clathrin pits in cancer? This study used immunofluorescence combined with superresolution structured illumination microscopy (SR-SIM) on normal and cancerous prostate tissue to reveal novel details of clathrin structure and biology. We show clathrin (heavy-chain) plaques and pits, expression of adaptor protein 2 (a clathrin adaptor protein) and epidermal growth factor receptor (a receptor target for CME) at nanometer scale in human tissue, in situ, with immunofluorescence-SR-SIM. There is an increase in the size of the clathrin pits in high-grade cancer compared with low-grade or normal prostate tissue. These results demonstrate that SR-SIM can be used to identify protein structures at high resolution in clinical tissue sections and there is an increased cargo capacity due to the increase in the size of clathrin pits as a mechanism that facilitates aggressiveness of cancer. These results shed new light on the pathology of cancer and the role CME via clathrin may play in carcinogenesis.