新型镧系(III)/镓（III）金属冠，附加可见吸收有机增敏剂，用于活细胞的分子近红外成像

IF 7.6 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Chemical Science Pub Date : 2025-06-12 DOI:10.1039/d5sc01320h

Timothée Lathion, Julie Bourseguin, Svetlana V. Eliseeva, Matthias Zeller, Stéphane Petoud, Vincent L. Pecoraro

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引用次数: 0

摘要

生物样品在近红外（NIR）领域的光学成像由于减少光散射、吸收和自身荧光而成为非侵入性实验的主要兴趣。利用[Ln2Ga8(shi)8(mip)4]2−金属冠中的马来酰亚胺基团；Shi3−=水杨酸酯；Mip2−= 5-马来酰二苯二甲酸酯；Ln = NdIII, GdIII, ErIII， YbIII和YIII)，我们通过巯基- michael加成点击反应添加了可见吸收的巯基-香豆素(C)敏化剂，得到了功能化的[Ln2Ga8(shi)8(C-mip)4]2−MCs。利用该方法，NIR-emitting NdIII， ErIII和YbIII在可见光范围内（λexc = 420 nm）通过附加香豆素激发实现了敏化。用NdIII衍生物孵育的活HeLa细胞的近红外荧光显微镜证实了细胞中在1050-1080 nm范围内由4F3/2→4I11/2电子跃迁产生的信号的明确检测。这些结果表明，[Ln2Ga8(shi)8(mip)4]2−MC可以作为一种通用分子来调节[Ln2Ga8] MC家族的化学和光物理性质。

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Novel lanthanide(III)/gallium(III) metallacrowns with appended visible-absorbing organic sensitizers for molecular near-infrared imaging of living cells

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Novel lanthanide(III)/gallium(III) metallacrowns with appended visible-absorbing organic sensitizers for molecular near-infrared imaging of living cells

Optical imaging of biological samples in the near-infrared (NIR) domain is of major interest for non-invasive experiments due to reduced light scattering, absorption and autofluorescence. Taking advantage of the maleimide moieties in the [Ln₂Ga₈(shi)₈(mip)₄]²⁻ metallacrowns (MCs; shi³⁻ = salicylhydroximate; mip²⁻ = 5-maleimido-isophthalate; Ln = Nd^III, Gd^III, Er^III, Yb^III and Y^III), we appended visible-absorbing thiol-coumarin (C) sensitizers through a thiol-Michael addition click reaction, affording the functionalized [Ln₂Ga₈(shi)₈(C-mip)₄]²⁻ MCs. By using this approach, the sensitization of NIR-emitting Nd^III, Er^III and Yb^III was achieved upon excitation in the visible range (λ_exc = 420 nm) through the appended coumarins. NIR epifluorescence microscopy of living HeLa cells incubated with the Nd^III derivative confirmed unambiguous detection of the signal arising from the ⁴F_3/2 → ⁴I_11/2 electronic transition in the range of 1050–1080 nm in the cells. These results demonstrate that the [Ln₂Ga₈(shi)₈(mip)₄]²⁻ MC can be used as a versatile molecule for tuning the chemical and photophysical properties of the [Ln₂Ga₈] MC family.

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来源期刊

Chemical Science CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

4.80%

发文量

1352

审稿时长

2.1 months

期刊介绍： Chemical Science is a journal that encompasses various disciplines within the chemical sciences. Its scope includes publishing ground-breaking research with significant implications for its respective field, as well as appealing to a wider audience in related areas. To be considered for publication, articles must showcase innovative and original advances in their field of study and be presented in a manner that is understandable to scientists from diverse backgrounds. However, the journal generally does not publish highly specialized research.