Run-Ze Liu, Li-An Bai, Yi Luo, Pei Liu, Fang Hua, Wei Fan, Bing Fan
{"title":"基于下一代测序的根管感染肠球菌的存在：一项系统回顾和荟萃分析。","authors":"Run-Ze Liu, Li-An Bai, Yi Luo, Pei Liu, Fang Hua, Wei Fan, Bing Fan","doi":"10.1111/iej.14266","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Studies based on next-generation sequencing (NGS) have reported conflicting evidence regarding the presence of <i>Enterococcus</i> in root canal infections.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To determine whether <i>Enterococcus</i> prevalence differs among secondary root canal infection (SRCI), persistent root canal infection (PRCI) and primary root canal infection (PrRCI).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Literature search was carried out across databases (PubMed, Web of Science, Scopus and Excerpta Medica Database) and other online resources from 1 September 2005, to 24 October 2024. Studies were selected according to inclusion and exclusion criteria. Risk of bias was evaluated according to the JBI Prevalence Critical Appraisal Checklist. The selection of 28 studies focused on the main and secondary outcomes. Pooled estimates of Odds Ratio (OR), prevalence and 95% Confidence Interval (CI) were determined with a common effect or random effects model. Multivariate meta-regression models were fitted. Subgroup analyses were conducted based on sample collection method, region and NGS platform. Sensitivity analysis was conducted to further investigate the robustness of results and to explore plausible causes for heterogeneity.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Among 28 included studies, 16 studies reporting the detection rate of <i>Enterococcus</i> were incorporated in the meta-analysis. The detection rate of <i>Enterococcus</i> was significantly higher in SRCI than in PrRCI (OR = 2.691; 95% CI: 1.234–5.868; <i>p</i> = .013). The pooled prevalences were 0.400 (95% CI: 0.235–0.565) for SRCI, 0.444 (95% CI: 0.000–0.976) for PRCI and 0.278 (95% CI: 0.087–0.470) for PrRCI, respectively. Within the PrRCI group, the cryogenic pulverization (CP) method yielded a significantly higher detection rate (0.768; 95% CI: 0.642–0.894) than the paper points/hand or rotary instruments (PP) method (0.113; 95% CI: 0.045–0.180). The relative abundance of <i>Enterococcus</i> ranged from 0.01% to 18.88% in SRCI, 2% to 10.5% in PRCI and 0.0008% to 2.03% in PrRCI.</p>\n </section>\n \n <section>\n \n <h3> Discussion</h3>\n \n <p>Although a quantitative synthesis of <i>Enterococcus</i> prevalence has been conducted, the data of abundance remain insufficient in literatures.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>A higher prevalence of <i>Enterococcus</i> was correlated with SRCI and PRCI compared to PrRCI. Sample collection method had a significant impact on the prevalence of <i>Enterococcus</i> in the PrRCI context. <i>Enterococcus</i> seems not to be a predominant genus in most studies based on its relatively low abundance.</p>\n </section>\n \n <section>\n \n <h3> Registration</h3>\n \n <p>PROSPERO database CRD42024609984.</p>\n </section>\n </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 9","pages":"1331-1353"},"PeriodicalIF":7.1000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.14266","citationCount":"0","resultStr":"{\"title\":\"The presence of Enterococcus in root canal infections based on next-generation sequencing: A systematic review and meta-analysis\",\"authors\":\"Run-Ze Liu, Li-An Bai, Yi Luo, Pei Liu, Fang Hua, Wei Fan, Bing Fan\",\"doi\":\"10.1111/iej.14266\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Studies based on next-generation sequencing (NGS) have reported conflicting evidence regarding the presence of <i>Enterococcus</i> in root canal infections.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To determine whether <i>Enterococcus</i> prevalence differs among secondary root canal infection (SRCI), persistent root canal infection (PRCI) and primary root canal infection (PrRCI).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Literature search was carried out across databases (PubMed, Web of Science, Scopus and Excerpta Medica Database) and other online resources from 1 September 2005, to 24 October 2024. Studies were selected according to inclusion and exclusion criteria. Risk of bias was evaluated according to the JBI Prevalence Critical Appraisal Checklist. The selection of 28 studies focused on the main and secondary outcomes. Pooled estimates of Odds Ratio (OR), prevalence and 95% Confidence Interval (CI) were determined with a common effect or random effects model. Multivariate meta-regression models were fitted. Subgroup analyses were conducted based on sample collection method, region and NGS platform. Sensitivity analysis was conducted to further investigate the robustness of results and to explore plausible causes for heterogeneity.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Among 28 included studies, 16 studies reporting the detection rate of <i>Enterococcus</i> were incorporated in the meta-analysis. The detection rate of <i>Enterococcus</i> was significantly higher in SRCI than in PrRCI (OR = 2.691; 95% CI: 1.234–5.868; <i>p</i> = .013). The pooled prevalences were 0.400 (95% CI: 0.235–0.565) for SRCI, 0.444 (95% CI: 0.000–0.976) for PRCI and 0.278 (95% CI: 0.087–0.470) for PrRCI, respectively. Within the PrRCI group, the cryogenic pulverization (CP) method yielded a significantly higher detection rate (0.768; 95% CI: 0.642–0.894) than the paper points/hand or rotary instruments (PP) method (0.113; 95% CI: 0.045–0.180). The relative abundance of <i>Enterococcus</i> ranged from 0.01% to 18.88% in SRCI, 2% to 10.5% in PRCI and 0.0008% to 2.03% in PrRCI.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Discussion</h3>\\n \\n <p>Although a quantitative synthesis of <i>Enterococcus</i> prevalence has been conducted, the data of abundance remain insufficient in literatures.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>A higher prevalence of <i>Enterococcus</i> was correlated with SRCI and PRCI compared to PrRCI. 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The presence of Enterococcus in root canal infections based on next-generation sequencing: A systematic review and meta-analysis
Background
Studies based on next-generation sequencing (NGS) have reported conflicting evidence regarding the presence of Enterococcus in root canal infections.
Objective
To determine whether Enterococcus prevalence differs among secondary root canal infection (SRCI), persistent root canal infection (PRCI) and primary root canal infection (PrRCI).
Methods
Literature search was carried out across databases (PubMed, Web of Science, Scopus and Excerpta Medica Database) and other online resources from 1 September 2005, to 24 October 2024. Studies were selected according to inclusion and exclusion criteria. Risk of bias was evaluated according to the JBI Prevalence Critical Appraisal Checklist. The selection of 28 studies focused on the main and secondary outcomes. Pooled estimates of Odds Ratio (OR), prevalence and 95% Confidence Interval (CI) were determined with a common effect or random effects model. Multivariate meta-regression models were fitted. Subgroup analyses were conducted based on sample collection method, region and NGS platform. Sensitivity analysis was conducted to further investigate the robustness of results and to explore plausible causes for heterogeneity.
Results
Among 28 included studies, 16 studies reporting the detection rate of Enterococcus were incorporated in the meta-analysis. The detection rate of Enterococcus was significantly higher in SRCI than in PrRCI (OR = 2.691; 95% CI: 1.234–5.868; p = .013). The pooled prevalences were 0.400 (95% CI: 0.235–0.565) for SRCI, 0.444 (95% CI: 0.000–0.976) for PRCI and 0.278 (95% CI: 0.087–0.470) for PrRCI, respectively. Within the PrRCI group, the cryogenic pulverization (CP) method yielded a significantly higher detection rate (0.768; 95% CI: 0.642–0.894) than the paper points/hand or rotary instruments (PP) method (0.113; 95% CI: 0.045–0.180). The relative abundance of Enterococcus ranged from 0.01% to 18.88% in SRCI, 2% to 10.5% in PRCI and 0.0008% to 2.03% in PrRCI.
Discussion
Although a quantitative synthesis of Enterococcus prevalence has been conducted, the data of abundance remain insufficient in literatures.
Conclusion
A higher prevalence of Enterococcus was correlated with SRCI and PRCI compared to PrRCI. Sample collection method had a significant impact on the prevalence of Enterococcus in the PrRCI context. Enterococcus seems not to be a predominant genus in most studies based on its relatively low abundance.
期刊介绍：
The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted.
The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.
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