{"title":"一种新型延胡索多糖的提取、结构分析及生物活性研究","authors":"Si-qi Zhang, Si-mei Liu, Zi-long Chen, Ke Guo, Xin-xin Yang, Li-hua Zhang, Ya-feng Yan, Ting-ting Zhang, Peng Zhao","doi":"10.1111/cbdd.70115","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>This research extracted and purified polysaccharides from <i>Corydalis yanhusuo</i> residue. The structure of purified polysaccharides of <i>Corydalis yanhusuo</i> (CYP-1) was characterized by methods such as infrared spectroscopy, nuclear magnetic resonance, and monosaccharide composition analysis. In addition, the purified polysaccharides were subjected to preliminary studies in vitro for antioxidant and antitumor activities. The average molecular weight of CYP-1 was 1.427 × 10³ kDa. FT-IR analysis revealed that CYP-1 displayed characteristic absorption bands typical of polysaccharides. Furthermore, NMR spectroscopy suggested that the sugar residue units of CYP-1 consisted of →1)-α-D-Glcp-(4→, →6)-α-D-Galp-(1→, and α-D-Arap-(1→. In vitro bioactivity experiments revealed that CYP-1 exhibits antioxidant activity and effectively inhibits HepG2 cell proliferation with an IC<sub>50</sub> value of 1.381 mg/mL, suggesting its potential as a natural antioxidant and antitumor agent.</p>\n </div>","PeriodicalId":143,"journal":{"name":"Chemical Biology & Drug Design","volume":"105 6","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Extraction, Structural Analysis, and Bioactivity of a Novel Polysaccharide From Corydalis yanhusuo Residues\",\"authors\":\"Si-qi Zhang, Si-mei Liu, Zi-long Chen, Ke Guo, Xin-xin Yang, Li-hua Zhang, Ya-feng Yan, Ting-ting Zhang, Peng Zhao\",\"doi\":\"10.1111/cbdd.70115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>This research extracted and purified polysaccharides from <i>Corydalis yanhusuo</i> residue. The structure of purified polysaccharides of <i>Corydalis yanhusuo</i> (CYP-1) was characterized by methods such as infrared spectroscopy, nuclear magnetic resonance, and monosaccharide composition analysis. In addition, the purified polysaccharides were subjected to preliminary studies in vitro for antioxidant and antitumor activities. The average molecular weight of CYP-1 was 1.427 × 10³ kDa. FT-IR analysis revealed that CYP-1 displayed characteristic absorption bands typical of polysaccharides. Furthermore, NMR spectroscopy suggested that the sugar residue units of CYP-1 consisted of →1)-α-D-Glcp-(4→, →6)-α-D-Galp-(1→, and α-D-Arap-(1→. In vitro bioactivity experiments revealed that CYP-1 exhibits antioxidant activity and effectively inhibits HepG2 cell proliferation with an IC<sub>50</sub> value of 1.381 mg/mL, suggesting its potential as a natural antioxidant and antitumor agent.</p>\\n </div>\",\"PeriodicalId\":143,\"journal\":{\"name\":\"Chemical Biology & Drug Design\",\"volume\":\"105 6\",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Biology & Drug Design\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/cbdd.70115\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Biology & Drug Design","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cbdd.70115","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
本研究从延胡索渣中提取纯化多糖。采用红外光谱、核磁共振、单糖组成分析等方法对延胡索多糖(CYP-1)的结构进行了表征。此外,还对纯化后的多糖进行了体外抗氧化和抗肿瘤活性的初步研究。cyp1的平均分子量为1.427 × 10³kDa。红外光谱分析表明,cyp1具有多糖的特征吸收带。核磁共振分析表明,CYP-1的糖残基单元由→1)-α- d - glcp -(4→,→6)-α- d - galp -(1→,α-D-Arap-(1→)组成。体外生物活性实验表明,cyp1具有抗氧化活性,可有效抑制HepG2细胞增殖,IC50值为1.381 mg/mL,提示其具有天然抗氧化和抗肿瘤作用。
Extraction, Structural Analysis, and Bioactivity of a Novel Polysaccharide From Corydalis yanhusuo Residues
This research extracted and purified polysaccharides from Corydalis yanhusuo residue. The structure of purified polysaccharides of Corydalis yanhusuo (CYP-1) was characterized by methods such as infrared spectroscopy, nuclear magnetic resonance, and monosaccharide composition analysis. In addition, the purified polysaccharides were subjected to preliminary studies in vitro for antioxidant and antitumor activities. The average molecular weight of CYP-1 was 1.427 × 10³ kDa. FT-IR analysis revealed that CYP-1 displayed characteristic absorption bands typical of polysaccharides. Furthermore, NMR spectroscopy suggested that the sugar residue units of CYP-1 consisted of →1)-α-D-Glcp-(4→, →6)-α-D-Galp-(1→, and α-D-Arap-(1→. In vitro bioactivity experiments revealed that CYP-1 exhibits antioxidant activity and effectively inhibits HepG2 cell proliferation with an IC50 value of 1.381 mg/mL, suggesting its potential as a natural antioxidant and antitumor agent.
期刊介绍:
Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.