一种新型延胡索多糖的提取、结构分析及生物活性研究

IF 3.3 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Si-qi Zhang, Si-mei Liu, Zi-long Chen, Ke Guo, Xin-xin Yang, Li-hua Zhang, Ya-feng Yan, Ting-ting Zhang, Peng Zhao
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引用次数: 0

摘要

本研究从延胡索渣中提取纯化多糖。采用红外光谱、核磁共振、单糖组成分析等方法对延胡索多糖(CYP-1)的结构进行了表征。此外,还对纯化后的多糖进行了体外抗氧化和抗肿瘤活性的初步研究。cyp1的平均分子量为1.427 × 10³kDa。红外光谱分析表明,cyp1具有多糖的特征吸收带。核磁共振分析表明,CYP-1的糖残基单元由→1)-α- d - glcp -(4→,→6)-α- d - galp -(1→,α-D-Arap-(1→)组成。体外生物活性实验表明,cyp1具有抗氧化活性,可有效抑制HepG2细胞增殖,IC50值为1.381 mg/mL,提示其具有天然抗氧化和抗肿瘤作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extraction, Structural Analysis, and Bioactivity of a Novel Polysaccharide From Corydalis yanhusuo Residues

This research extracted and purified polysaccharides from Corydalis yanhusuo residue. The structure of purified polysaccharides of Corydalis yanhusuo (CYP-1) was characterized by methods such as infrared spectroscopy, nuclear magnetic resonance, and monosaccharide composition analysis. In addition, the purified polysaccharides were subjected to preliminary studies in vitro for antioxidant and antitumor activities. The average molecular weight of CYP-1 was 1.427 × 10³ kDa. FT-IR analysis revealed that CYP-1 displayed characteristic absorption bands typical of polysaccharides. Furthermore, NMR spectroscopy suggested that the sugar residue units of CYP-1 consisted of →1)-α-D-Glcp-(4→, →6)-α-D-Galp-(1→, and α-D-Arap-(1→. In vitro bioactivity experiments revealed that CYP-1 exhibits antioxidant activity and effectively inhibits HepG2 cell proliferation with an IC50 value of 1.381 mg/mL, suggesting its potential as a natural antioxidant and antitumor agent.

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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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