{"title":"建立一种方便、快速筛选的斑马鱼模型来研究尿毒症毒素的致死效应和免疫功能低下状态。","authors":"Pema Jo, Rui Zhang, Yuhua Zhang, Zhilian Li, Yanhua Wu, Shuqiong Zhang, Tsring Lhamo, Yuanhan Chen","doi":"10.1080/0886022X.2025.2514183","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Uremia is characterized by high mortality and immune dysfunction owing to the accumulation of uremic toxins. Traditional rodent models are complex and time consuming. This study aimed to develop a simple and rapid zebrafish model for investigating the toxicological effects of uremic toxins.</p><p><strong>Methods: </strong>Uremic solutions containing small (SUT) and medium-to-large (MLUT) uremic toxins were prepared using waste solution of peritoneal dialysis by a dialysis method. Wild-type zebrafish larvae were used to assess mortality, whereas transgenic (TG) (zlyz:EGFP) zebrafish, with macrophages labeled with green fluorescent protein, were used to evaluate immune status through macrophage migration assays.</p><p><strong>Results: </strong>The mortality rates were significantly higher in the SUT- and MLUT-treated groups than in the controls, with SUT showing the strongest lethal effect. Macrophage migration was significantly inhibited in both SUT- and MLUT-treated groups, indicating an immunocompromise. This model effectively mimicked the lethal and immunosuppressive effects of uremia.</p><p><strong>Conclusion: </strong>This zebrafish model provides a simple and rapid platform for studying the toxicological effects of uremic toxins and evaluating potential therapeutic interventions.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2514183"},"PeriodicalIF":3.0000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150638/pdf/","citationCount":"0","resultStr":"{\"title\":\"Development of a convenient and rapid screening zebrafish model to investigate lethal effects and immunocompromised states of uremic toxins.\",\"authors\":\"Pema Jo, Rui Zhang, Yuhua Zhang, Zhilian Li, Yanhua Wu, Shuqiong Zhang, Tsring Lhamo, Yuanhan Chen\",\"doi\":\"10.1080/0886022X.2025.2514183\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Uremia is characterized by high mortality and immune dysfunction owing to the accumulation of uremic toxins. Traditional rodent models are complex and time consuming. This study aimed to develop a simple and rapid zebrafish model for investigating the toxicological effects of uremic toxins.</p><p><strong>Methods: </strong>Uremic solutions containing small (SUT) and medium-to-large (MLUT) uremic toxins were prepared using waste solution of peritoneal dialysis by a dialysis method. Wild-type zebrafish larvae were used to assess mortality, whereas transgenic (TG) (zlyz:EGFP) zebrafish, with macrophages labeled with green fluorescent protein, were used to evaluate immune status through macrophage migration assays.</p><p><strong>Results: </strong>The mortality rates were significantly higher in the SUT- and MLUT-treated groups than in the controls, with SUT showing the strongest lethal effect. Macrophage migration was significantly inhibited in both SUT- and MLUT-treated groups, indicating an immunocompromise. This model effectively mimicked the lethal and immunosuppressive effects of uremia.</p><p><strong>Conclusion: </strong>This zebrafish model provides a simple and rapid platform for studying the toxicological effects of uremic toxins and evaluating potential therapeutic interventions.</p>\",\"PeriodicalId\":20839,\"journal\":{\"name\":\"Renal Failure\",\"volume\":\"47 1\",\"pages\":\"2514183\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150638/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Renal Failure\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/0886022X.2025.2514183\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"UROLOGY & NEPHROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Renal Failure","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/0886022X.2025.2514183","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/8 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
Development of a convenient and rapid screening zebrafish model to investigate lethal effects and immunocompromised states of uremic toxins.
Background: Uremia is characterized by high mortality and immune dysfunction owing to the accumulation of uremic toxins. Traditional rodent models are complex and time consuming. This study aimed to develop a simple and rapid zebrafish model for investigating the toxicological effects of uremic toxins.
Methods: Uremic solutions containing small (SUT) and medium-to-large (MLUT) uremic toxins were prepared using waste solution of peritoneal dialysis by a dialysis method. Wild-type zebrafish larvae were used to assess mortality, whereas transgenic (TG) (zlyz:EGFP) zebrafish, with macrophages labeled with green fluorescent protein, were used to evaluate immune status through macrophage migration assays.
Results: The mortality rates were significantly higher in the SUT- and MLUT-treated groups than in the controls, with SUT showing the strongest lethal effect. Macrophage migration was significantly inhibited in both SUT- and MLUT-treated groups, indicating an immunocompromise. This model effectively mimicked the lethal and immunosuppressive effects of uremia.
Conclusion: This zebrafish model provides a simple and rapid platform for studying the toxicological effects of uremic toxins and evaluating potential therapeutic interventions.
期刊介绍：
Renal Failure primarily concentrates on acute renal injury and its consequence, but also addresses advances in the fields of chronic renal failure, hypertension, and renal transplantation. Bringing together both clinical and experimental aspects of renal failure, this publication presents timely, practical information on pathology and pathophysiology of acute renal failure; nephrotoxicity of drugs and other substances; prevention, treatment, and therapy of renal failure; renal failure in association with transplantation, hypertension, and diabetes mellitus.
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