从基因到功能：细胞色素c亚硝酸盐还原酶在硝酸还原成铵过程中的调控、成熟和进化。

IF 3.7 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied and Environmental Microbiology Pub Date : 2025-07-23 Epub Date: 2025-06-09 DOI:10.1128/aem.00292-25

Krystina Hird, Julius O Campeciño, Eric L Hegg

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引用次数: 0

摘要

异化硝酸还原为铵的途径将硝酸盐转化为铵，这是全球氮循环中的一个重要反应。该途径的第二步是由细胞色素c亚硝酸盐还原酶（NrfA）完成的，NrfA是一种可溶性的细胞色素，负责将亚硝酸盐还原为铵。五胺NrfA在其喹啉氧化酶伙伴的帮助下，在单个活性位点催化亚硝酸盐的6电子和8质子还原。在这篇综述中，我们讨论了我们目前对以下方面的理解：(i) NrfA及其氧化还原伙伴的结构、同源性和进化，（ii） NrfA操纵子的调控，以及（iii） NrfA蛋白通过独特的细胞色素成熟途径成熟。

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From genes to function: regulation, maturation, and evolution of cytochrome c nitrite reductase in nitrate reduction to ammonium.

The dissimilatory nitrate reduction to ammonium pathway converts nitrate to ammonium, a vital reaction in the global nitrogen cycle. The second step of the pathway is performed by cytochrome c nitrite reductase (NrfA), a soluble, periplasmic cytochrome responsible for the reduction of nitrite to ammonium. The pentaheme NrfA catalyzes this six-electron and eight-proton reduction of nitrite at a single active site with the help of its quinol oxidase partners. In this review, we discuss our current understanding of (i) the structure, homology, and evolution of both NrfA and its redox partners, (ii) the regulation of the nrf operon, and (iii) the maturation of NrfA proteins via unique cytochrome maturation pathways.

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来源期刊

Applied and Environmental Microbiology 生物-生物工程与应用微生物

CiteScore

7.70

自引率

2.30%

发文量

730

审稿时长

1.9 months

期刊介绍： Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.