利用荚膜甲基球菌（Bath）生物质和工程解脂耶氏菌将甲烷转化为角黄素的两步法研究

IF 3.1 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-06-09 DOI:10.1002/biot.70043

Maria O. Taratynova, Ivan M. Tarasov, Iuliia M. Fedyaeva, Dmitry A. Dementev, Veronika A. Gorchakova, Margarita A. Tarasova, Alexander S. Fedorov, Tigran V. Yuzbashev, Sergey P. Sineoky, Evgeniya Y. Yuzbasheva

{"title":"利用荚膜甲基球菌（Bath）生物质和工程解脂耶氏菌将甲烷转化为角黄素的两步法研究","authors":"Maria O. Taratynova, Ivan M. Tarasov, Iuliia M. Fedyaeva, Dmitry A. Dementev, Veronika A. Gorchakova, Margarita A. Tarasova, Alexander S. Fedorov, Tigran V. Yuzbashev, Sergey P. Sineoky, Evgeniya Y. Yuzbasheva","doi":"10.1002/biot.70043","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Methane biotransformation is gaining attention for the bio-industry and environmental protection. This study presents an approach for converting methane to canthaxanthin using <i>Methylococcus capsulatus</i> biomass and <i>Yarrowia lipolytica</i>. <i>Y. lipolytica</i> was engineered to introduce the canthaxanthin biosynthesis pathway, increase the acetyl-CoA flux, and boost hexose catabolism. In methanotroph-derived medium with molasses, canthaxanthin titer reached 1.2 g/L, advancing cost-effective, sustainable bioproduction.</p>\n </div>","PeriodicalId":134,"journal":{"name":"Biotechnology Journal","volume":"20 6","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Two-Step Process for Converting Methane to Canthaxanthin Using Methylococcus capsulatus (Bath) Biomass and Engineered Yarrowia lipolytica\",\"authors\":\"Maria O. Taratynova, Ivan M. Tarasov, Iuliia M. Fedyaeva, Dmitry A. Dementev, Veronika A. Gorchakova, Margarita A. Tarasova, Alexander S. Fedorov, Tigran V. Yuzbashev, Sergey P. Sineoky, Evgeniya Y. Yuzbasheva\",\"doi\":\"10.1002/biot.70043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Methane biotransformation is gaining attention for the bio-industry and environmental protection. This study presents an approach for converting methane to canthaxanthin using <i>Methylococcus capsulatus</i> biomass and <i>Yarrowia lipolytica</i>. <i>Y. lipolytica</i> was engineered to introduce the canthaxanthin biosynthesis pathway, increase the acetyl-CoA flux, and boost hexose catabolism. In methanotroph-derived medium with molasses, canthaxanthin titer reached 1.2 g/L, advancing cost-effective, sustainable bioproduction.</p>\\n </div>\",\"PeriodicalId\":134,\"journal\":{\"name\":\"Biotechnology Journal\",\"volume\":\"20 6\",\"pages\":\"\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/biot.70043\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Journal","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/biot.70043","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

甲烷的生物转化在生物产业和环境保护方面受到越来越多的关注。本研究提出了一种利用荚膜甲基球菌和多脂耶氏菌将甲烷转化为角黄素的方法。脂解菌被设计为引入角黄素生物合成途径，增加乙酰辅酶a通量，促进己糖分解代谢。在含糖蜜的甲烷氧化菌衍生培养基中，角黄素滴度达到1.2 g/L，促进了经济、可持续的生物生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Two-Step Process for Converting Methane to Canthaxanthin Using Methylococcus capsulatus (Bath) Biomass and Engineered Yarrowia lipolytica

查看原文本刊更多论文

A Two-Step Process for Converting Methane to Canthaxanthin Using Methylococcus capsulatus (Bath) Biomass and Engineered Yarrowia lipolytica

Methane biotransformation is gaining attention for the bio-industry and environmental protection. This study presents an approach for converting methane to canthaxanthin using Methylococcus capsulatus biomass and Yarrowia lipolytica. Y. lipolytica was engineered to introduce the canthaxanthin biosynthesis pathway, increase the acetyl-CoA flux, and boost hexose catabolism. In methanotroph-derived medium with molasses, canthaxanthin titer reached 1.2 g/L, advancing cost-effective, sustainable bioproduction.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.