异亮氨酸和亮氨酸被酮酸替代导致中国仓鼠卵巢细胞α-羟基酸的形成增加

IF 3.2 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-06-09 DOI:10.1002/biot.70041

Philipp Reifenberg, Lara Rosenberger, Maxime Le Mignon, Aline Zimmer

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引用次数: 0

摘要

酮异亮氨酸和酮亮氨酸是CHO细胞产生生物治疗药物的生物可利用氨基酸前体。在CHOK1 GS细胞的间歇培养中添加它们后，三种支链酮酸的代谢物的定量显示它们的脱羧产物减少，但α-羟基酸的积累。后者对细胞生长和生产力的影响较小。l -乳酸脱氢酶是形成缬氨酸α-羟基酸的关键酶。

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Replacement of Isoleucine and Leucine by their Keto Acids leads to increased formation of α-Hydroxy Acids in Chinese Hamster Ovary cells

Keto isoleucine and keto leucine are bioavailable amino acid precursors in CHO cells producing biotherapeutics. Following their supplementation in a fed-batch culture with CHOK1 GS cells, quantitation of metabolites derived from the three branched-chain keto acids revealed a decrease of their decarboxylation products, but an accumulation of their α-hydroxy acids. The latter had minor effects on cell growth and productivity. L-lactate dehydrogenase was identified as the key enzyme forming valine's α-hydroxy acid.

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.