通过对加拿大市政和机构废水样本的高通量测序鉴定循环人类乳头瘤病毒类型。

IF 3.7 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Applied and Environmental Microbiology Pub Date : 2025-07-23 Epub Date: 2025-06-05 DOI:10.1128/aem.00348-25
Shayna J Giesbrecht, Samantha J Krosta, Rebecca Fox, Kurt Kolsun, Zoe Quill, Suzanne Gibbons, Aida Sivro, Paul Sandstrom, Chand S Mangat, Michael G Becker
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引用次数: 0

摘要

人乳头瘤病毒(hpv)是一种感染人类粘膜和皮肤组织的多种病毒,在全球范围内普遍存在。有几种类型由于与宫颈癌、肛门癌、阴茎癌和口咽癌相关而被列为高危类型。鉴于其广泛流行和临床监测的局限性,HPV是基于废水的检测(WBT)的理想候选者,作为一种表征循环HPV类型的方法。为了实现这一目标,我们利用基于illumina的高通量扩增子测序,靶向从两个城市处理厂、两个城镇和三个教养设施收集的废水样本中HPV的保守GP5+/GP6+位点。与机构环境相比,较大的人口集水区在废水中显示出更大的HPV类型多样性,反映了社区组成和暴露的差异。总共检测到24种不同的HPV类型,均属于α -乳头瘤病毒属,包括高风险型和低风险型,未知风险型,以及皮肤型和粘膜型。其中包括六种疫苗可预防的类型,包括HPV-16和HPV-18,以及在临床环境中未常规监测的可能致癌类型,如HPV-67, HPV-69和HPV-73。这些发现证明了WBT作为临床测试的补充工具的效用,为癌症预防策略和评估疫苗接种计划的影响提供了重要的见解。重要性:人乳头瘤病毒(HPV)相关癌症,包括宫颈癌、肛门癌、阴茎癌和口咽癌,给卫生保健系统带来了重大的经济负担,每年用于治疗和管理的费用高达数十亿美元。本研究报告了加拿大废水中HPV的检测,为社区内HPV循环提供了新的见解。研究结果强调了疫苗靶向的高危HPV类型的持续流行以及可能的致癌类型的循环,例如HPV-67, HPV-69和HPV-73,这些类型未包括在当前的临床测试算法中。基于废水的HPV监测可以通过获取临床环境中代表性不足的人群的数据来补充临床检测。更清楚地了解流行的HPV类型可以支持公共卫生工作,以促进癌症筛查,监测疫苗接种运动,并解决预防战略方面的差距。基于废水的检测系统的持续发展预示着对社区健康进行公正监测的前景光明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of circulating human papillomavirus types through high-throughput sequencing of Canadian municipal and institutional wastewater samples.

Human papillomaviruses (HPVs) are a diverse group of viruses that infect human mucosal and cutaneous tissues, with a ubiquitous global distribution. Several types are classified as high-risk due to their association with cervical, anal, penile, and oropharyngeal cancers. Given its widespread prevalence and the limitations of clinical surveillance, HPV is an ideal candidate for wastewater-based testing (WBT), as a way to characterize circulating HPV types. Toward this goal, we utilized high-throughput Illumina-based amplicon sequencing targeting the conserved GP5+/GP6+ sites of HPV in wastewater samples collected from two urban treatment plants, two town sites, and three correctional facilities. Larger population catchments displayed greater diversity of HPV types in wastewater compared to institutional settings, reflecting differences in community composition and exposure. A total of 24 distinct HPV types were detected, all belonging to the alpha-papillomavirus genus, encompassing high- and low-risk types, types of unknown risk, and both cutaneous and mucosal types. Among these were six vaccine-preventable types, including HPV-16 and HPV-18, as well as probably carcinogenic types not routinely monitored in clinical settings, such as HPV-67, HPV-69, and HPV-73. These findings demonstrate the utility of WBT as a complementary tool to clinical testing, providing critical insights to inform cancer prevention strategies and evaluate the impact of vaccination programs.

Importance: Human papillomavirus (HPV)-related cancers, including cervical, anal, penile, and oropharyngeal cancers, impose a significant economic burden on healthcare systems, with billions spent annually on treatment and management. This study reports the detection of HPV in Canadian wastewater, providing new insights into HPV circulation within communities. The findings highlight the ongoing prevalence of high-risk HPV types targeted by vaccines and the circulation of probable carcinogenic types, such as HPV-67, HPV-69, and HPV-73, which are not included in current clinical testing algorithms. Wastewater-based surveillance of HPV could complement clinical testing by capturing data on populations typically underrepresented in clinical settings. A clearer understanding of circulating HPV types can support public health efforts to promote cancer screening, monitor vaccination campaigns, and address gaps in prevention strategies. The continued development of wastewater-based testing systems hints toward a promising future for unbiased monitoring of community health.

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来源期刊
Applied and Environmental Microbiology
Applied and Environmental Microbiology 生物-生物工程与应用微生物
CiteScore
7.70
自引率
2.30%
发文量
730
审稿时长
1.9 months
期刊介绍: Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.
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