如何给玉米授粉（玉米）。

Cold Spring Harbor protocols Pub Date : 2025-06-03 DOI:10.1101/pdb.prot108638

Sarah L Fitzsimmons, Miriam Nancy Salazar-Vidal, Zachary B Traylor, Melissa A Draves, Sherry Flint-Garcia

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引用次数: 0

摘要

玉米是一种重要的经济作物，也是遗传学研究中经常使用的模式生物。控制授粉对这些研究的实施至关重要。本协议概述了计划、建立和进行人工授粉的基本步骤。此外，还详细介绍了多次传粉的花粉扩展方法、传粉类型及其相应的标记方案。提供了故障排除指南，提供了在进行手动授粉时可能遇到的常见问题的解决方案。尽管生长条件和种质资源可能会导致本协议的轻微偏差，但基本原则可以适用于任何玉米研究计划。

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How to Pollinate Corn (Zea mays).

Corn, or maize, is an economically important crop and frequently used model organism for genetic studies. Controlled pollinations are essential to the execution of these studies. This protocol outlines the basic steps in planning, setting up, and making manual pollinations. In addition, a method of extending pollen for multiple pollinations, as well as types of pollinations and their respective labeling schemes, are detailed. A troubleshooting guide is provided with solutions for common problems one might encounter when making manual pollinations. Although growing conditions and germplasm may cause slight deviations from this protocol, the basic principles can be applied to any corn research program.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.