基于“DMT-On”策略的RNA化学磷酸化试剂。

IF 5 1区化学 Q1 CHEMISTRY, ORGANIC

Organic Letters Pub Date : 2025-06-13 Epub Date: 2025-06-03 DOI:10.1021/acs.orglett.5c01932

Coelio Vallée, Jawad Belkheir, Nina Gonnet, Rémy Lartia

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引用次数: 0

摘要

我们提出了一种新的围绕二羟基丙酮核心构建的磷酸化试剂。DNA和更有趣的是，RNA可以很容易地被磷酸化，通过使用一种类似于合成未修饰核酸的常规方法。因此，与二甲氧基三甲基相关的众所周知的优势（通过三甲基阳离子测定法在树脂上监测，高效液相色谱延迟保留，简单的脱保护步骤，以及使用低聚实验室常见的设备）得以保持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

An RNA Chemical Phosphorylation Reagent Based on the "DMT-On" Strategy.

查看原文本刊更多论文

An RNA Chemical Phosphorylation Reagent Based on the "DMT-On" Strategy.

We present a new phosphorylation reagent built around a dihydroxyacetone core. DNA and, more interestingly, RNA can be easily phosphorylated by using a protocol similar to that routinely employed in the synthesis of unmodified nucleic acids. The well-known advantages associated with the dimethoxytrityl group (on-resin monitoring by trityl cation assay, delayed retention on HPLC, simple deprotection steps, and the use of equipment commonly found in oligo laboratories) are thus maintained.

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来源期刊

Organic Letters 化学-有机化学

CiteScore

9.30

自引率

11.50%

发文量

1607

审稿时长

1.5 months

期刊介绍： Organic Letters invites original reports of fundamental research in all branches of the theory and practice of organic, physical organic, organometallic,medicinal, and bioorganic chemistry. Organic Letters provides rapid disclosure of the key elements of significant studies that are of interest to a large portion of the organic community. In selecting manuscripts for publication, the Editors place emphasis on the originality, quality and wide interest of the work. Authors should provide enough background information to place the new disclosure in context and to justify the rapid publication format. Back-to-back Letters will be considered. Full details should be reserved for an Article, which should appear in due course.