绘制一种新出现的人类病原体小叶虫的基因组序列。

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-05-31 DOI:10.1016/j.ygeno.2025.111062

Liping Liu , Qiuping Wang , Hui Wang , Shufen Quan , Mian Wang , Dechun Xie , Yu'e Hao

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引用次数: 0

摘要

小野蝇被认为是造成严重感染的一种新出现的人类病原体。然而，缺乏这种致病真菌的基因组序列阻碍了其致病机制的分子和遗传学研究。在这项研究中，我们报道了从临床样本中分离的K. ohmeri 3873基因组草图。基因组大小为13.86 Mb，包括3734个推测基因，54.22 %的分散重复序列和串联重复序列。进一步的生物信息学分析发现了159个碳水化合物活性酶、19个CYP基因和44个次级代谢物基因。共鉴定出657个致病性相关基因（包括多药耐药基因）和216个真菌毒力因子（与人类疾病相关的基因172个），比其他7个菌株多，提示该菌株有可能成为新兴的人类病原体。本研究为进一步开展霍默氏弧菌的生物学、分子发病机制和毒力研究奠定了基础。

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Draft genome sequence of Kodamaea ohmeri, an emerging human pathogen

Kodamaea ohmeri is recognized as an emerging human pathogen responsible for severe infections. However, the lack of genome sequence for this pathogenic fungus hampers molecular and genetic investigations of its pathogenic mechanism. In this study, we reported the draft genome of K. ohmeri 3873 isolated from clinical samples. The genome has a size of 13.86 Mb, including 3734 putative genes with 54.22 % of dispersed and tandem repeat sequences. Further bioinformatics analysis found 159 carbohydrate-active enzymes, 19 CYP genes and 44 secondary metabolite genes in K. ohmeri genome. A total of 657 pathogenicity related genes (including multidrug resistance genes) and 216 fungal virulence factors (172 genes associated with human diseases) were identified, which were more than those of other seven strains, suggesting the potential of K. ohmeri as an emerging human pathogen. This study provides a foundation for future works on the biology, molecular pathogenesis, and virulence of K. ohmeri.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.