Thao V. Nguyen , Andrea C. Alfaro , Thanh Nha Ngo , Anu Kumar , David Beale
{"title":"多平台代谢组学方法研究急性热应激对新西兰黑足鲍鱼的影响","authors":"Thao V. Nguyen , Andrea C. Alfaro , Thanh Nha Ngo , Anu Kumar , David Beale","doi":"10.1016/j.fishres.2025.107426","DOIUrl":null,"url":null,"abstract":"<div><div>The rise in ocean temperatures and the increasing frequency of marine heatwave events pose significant threats to shellfish fisheries and marine ecosystems worldwide. In New Zealand, natural abalone (<em>Haliotis iris</em>) populations have recently experienced elevated mortalities, particularly during summer months, due to these extreme thermal events. This study employed a multi-platform metabolomics and lipidomics approach using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) approach, to investigate the metabolic responses of adult abalone to three temperature treatments (14, 19 and 24 °C) over a 24-hour exposure period in a controlled laboratory experiment. A total of 34 metabolites and 34 lipids were identified in foot muscle tissue as significantly altered across temperature treatments, with the majority exhibiting higher concentrations at 24 °C compared to the lower temperature groups. These metabolic shifts indicate cellular and oxidative damage induced by acute thermal stress, along with evidence of metabolic depression, disruptions in energy metabolism, lipid metabolism, osmotic regulation, and alterations in membrane composition and stability. Notably, the decrease in aspartic acid levels, alongside changes in tricarboxylic acid (TCA) cycle intermediates, suggests the activation of the aspartate–succinate pathway as an alternative energy production mechanism under heat stress. Overall, this study provides valuable insights into the metabolic and lipidomic responses of abalone to rising ocean temperatures, enhancing our understanding of how marine organisms respond to climate change and extreme thermal events.</div></div>","PeriodicalId":50443,"journal":{"name":"Fisheries Research","volume":"287 ","pages":"Article 107426"},"PeriodicalIF":2.3000,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A multi-platform metabolomics approach to investigate the effects of acute thermal stress on New Zealand black-footed abalone (Haliotis iris)\",\"authors\":\"Thao V. Nguyen , Andrea C. Alfaro , Thanh Nha Ngo , Anu Kumar , David Beale\",\"doi\":\"10.1016/j.fishres.2025.107426\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The rise in ocean temperatures and the increasing frequency of marine heatwave events pose significant threats to shellfish fisheries and marine ecosystems worldwide. In New Zealand, natural abalone (<em>Haliotis iris</em>) populations have recently experienced elevated mortalities, particularly during summer months, due to these extreme thermal events. This study employed a multi-platform metabolomics and lipidomics approach using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) approach, to investigate the metabolic responses of adult abalone to three temperature treatments (14, 19 and 24 °C) over a 24-hour exposure period in a controlled laboratory experiment. A total of 34 metabolites and 34 lipids were identified in foot muscle tissue as significantly altered across temperature treatments, with the majority exhibiting higher concentrations at 24 °C compared to the lower temperature groups. These metabolic shifts indicate cellular and oxidative damage induced by acute thermal stress, along with evidence of metabolic depression, disruptions in energy metabolism, lipid metabolism, osmotic regulation, and alterations in membrane composition and stability. Notably, the decrease in aspartic acid levels, alongside changes in tricarboxylic acid (TCA) cycle intermediates, suggests the activation of the aspartate–succinate pathway as an alternative energy production mechanism under heat stress. Overall, this study provides valuable insights into the metabolic and lipidomic responses of abalone to rising ocean temperatures, enhancing our understanding of how marine organisms respond to climate change and extreme thermal events.</div></div>\",\"PeriodicalId\":50443,\"journal\":{\"name\":\"Fisheries Research\",\"volume\":\"287 \",\"pages\":\"Article 107426\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fisheries Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0165783625001638\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fisheries Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165783625001638","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
A multi-platform metabolomics approach to investigate the effects of acute thermal stress on New Zealand black-footed abalone (Haliotis iris)
The rise in ocean temperatures and the increasing frequency of marine heatwave events pose significant threats to shellfish fisheries and marine ecosystems worldwide. In New Zealand, natural abalone (Haliotis iris) populations have recently experienced elevated mortalities, particularly during summer months, due to these extreme thermal events. This study employed a multi-platform metabolomics and lipidomics approach using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) approach, to investigate the metabolic responses of adult abalone to three temperature treatments (14, 19 and 24 °C) over a 24-hour exposure period in a controlled laboratory experiment. A total of 34 metabolites and 34 lipids were identified in foot muscle tissue as significantly altered across temperature treatments, with the majority exhibiting higher concentrations at 24 °C compared to the lower temperature groups. These metabolic shifts indicate cellular and oxidative damage induced by acute thermal stress, along with evidence of metabolic depression, disruptions in energy metabolism, lipid metabolism, osmotic regulation, and alterations in membrane composition and stability. Notably, the decrease in aspartic acid levels, alongside changes in tricarboxylic acid (TCA) cycle intermediates, suggests the activation of the aspartate–succinate pathway as an alternative energy production mechanism under heat stress. Overall, this study provides valuable insights into the metabolic and lipidomic responses of abalone to rising ocean temperatures, enhancing our understanding of how marine organisms respond to climate change and extreme thermal events.
期刊介绍:
This journal provides an international forum for the publication of papers in the areas of fisheries science, fishing technology, fisheries management and relevant socio-economics. The scope covers fisheries in salt, brackish and freshwater systems, and all aspects of associated ecology, environmental aspects of fisheries, and economics. Both theoretical and practical papers are acceptable, including laboratory and field experimental studies relevant to fisheries. Papers on the conservation of exploitable living resources are welcome. Review and Viewpoint articles are also published. As the specified areas inevitably impinge on and interrelate with each other, the approach of the journal is multidisciplinary, and authors are encouraged to emphasise the relevance of their own work to that of other disciplines. The journal is intended for fisheries scientists, biological oceanographers, gear technologists, economists, managers, administrators, policy makers and legislators.