放射治疗联合5-氨基乙酰丙酸对人原发性脑膜瘤细胞的影响

IF 3.2 3区医学 Q2 ONCOLOGY

Clinical oncology Pub Date : 2025-05-09 DOI:10.1016/j.clon.2025.103863

S. Ebrahimi , M. Rezaei Arjomand , A. Tabibkhooei , M. Khaleghi Ghadiri , W. Stummer , A. Gorji

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引用次数: 0

摘要

目的放射治疗（RT）是某些脑膜瘤的标准治疗方法；然而，它并不总是取得有利的结果。因此，发现能够增强脑膜瘤放射敏感性的新药物具有相当大的意义。证据表明，5-氨基乙酰丙酸（5-ALA）同时作为光敏剂和放射敏剂。本研究旨在评估5-ALA联合RT治疗原发性脑膜瘤细胞的疗效，这些细胞来自8例手术中获得的患者。方法采用免疫细胞化学方法对原发性脑膜瘤细胞进行特异性标记。用WST-1法评估5-ALA治疗后细胞增殖情况，不论有无RT。荧光法测定原卟啉IX的积累。结果我们的体外研究表明，虽然5-ALA单独使用会降低细胞增殖，但5-ALA和RT联合使用对细胞生长的抑制作用更为明显。5-ALA的细胞毒性剂量在不同脑膜瘤患者的细胞中有显著差异。此外，研究发现5-ALA可有效诱导脑膜瘤细胞内原卟啉IX的积累，原卟啉IX是一种高度促凋亡的荧光光敏剂。结论5-ALA显著增强了RT的抗肿瘤作用，为进一步探索5-ALA联合RT治疗脑膜瘤提供了新的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Radiation Therapy Combined With 5-Aminolevulinic Acid: Effect on Primary Human Meningioma Cells

Aims

Radiation therapy (RT) is a standard treatment for certain meningiomas; however, it does not consistently achieve favorable favourable outcomes. Therefore, discovering new agents that can enhance the radiosensitivity of meningiomas is of considerable interest. Evidence suggests that 5-aminolevulinic acid (5-ALA) acts both as a photosensitiszer and a radiosensitiszer. This study aimed to evaluate the efficacy of combining 5-ALA with RT on primary meningioma cells, which were obtained from eight patients during surgery.

Methods

Primary meningioma cells were characterized by immunocytochemistry using specific markers. Cell proliferation following treatment with 5-ALA, with or without RT, was evaluated using the WST-1 assay. Accumulation of protoporphyrin IX was evaluated fluorometrically.

Results

Our in vitro investigations indicate that whilste 5-ALA alone reduced cell proliferation, the combination of 5-ALA and RT resulted in more pronounced cell growth inhibition. The cytotoxic doses of 5-ALA markedly varied amongst cells from different meningioma patients. Furthermore, the study revealed that 5-ALA effectively induced the accumulation of protoporphyrin IX, a highly pro-apoptotic and fluorescent photosensitiszer, within the meningioma cells.

Conclusion

Our data suggest that 5-ALA significantly enhances the antitumor antitumour effects of RT, offering a new option for further exploration of 5-ALA combined with RT as a valuable strategy for meningioma treatment.

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来源期刊

Clinical oncology 医学-肿瘤学

CiteScore

5.20

自引率

8.80%

发文量

332

审稿时长

40 days

期刊介绍： Clinical Oncology is an International cancer journal covering all aspects of the clinical management of cancer patients, reflecting a multidisciplinary approach to therapy. Papers, editorials and reviews are published on all types of malignant disease embracing, pathology, diagnosis and treatment, including radiotherapy, chemotherapy, surgery, combined modality treatment and palliative care. Research and review papers covering epidemiology, radiobiology, radiation physics, tumour biology, and immunology are also published, together with letters to the editor, case reports and book reviews.