Mehdi Moeil, Majid Tafrihi, Ehsan Nazifi, Maryam Radfar
{"title":"桃叶乙酸乙酯提取物对MCF-7细胞诱导凋亡的体外研究","authors":"Mehdi Moeil, Majid Tafrihi, Ehsan Nazifi, Maryam Radfar","doi":"10.18502/ajmb.v17i2.18564","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Teucrium persicum</i> (<i>T. persicum</i>) is a well-known Iranian endemic plant that grows in the southern regions of Iran. It is used as a tea to treat abdominal pains, hyperlipidemia, and diabetes in traditional Iranian medicine. It has been previously found that the methanolic extract of <i>T. persicum</i> exerts significant cytotoxicity and inhibitory effects on different cancer cells. This study aimed to investigate the effects of ethyl acetate extract of <i>T. persicum</i> on MCF-7 cells.</p><p><strong>Methods: </strong>The experiments included MTT, DAPI staining, and investigating the expression of <i>BAX</i> and <i>BCL2</i> genes. The extract had a significant cytotoxic effect on MCF-7 cells, with an IC<sub>50</sub> value of 50 <i>μg/ml</i> for 48 <i>hr</i>.</p><p><strong>Results: </strong>DAPI staining assays showed that the extract induced morphological changes, chromatin condensation, and nuclear fragmentation. Additionally, the ethyl acetate extract induced the expression of <i>BAX</i> and down-regulated <i>BCL2</i> genes.</p><p><strong>Conclusion: </strong>These findings suggest that <i>T. persicum</i> has strong cytotoxic properties and warrants further investigation.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"131-135"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123179/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Pro-Apoptosis Induction of <i>Teucrium persicum</i> Ethyl Acetate Extract on MCF-7 Cells: An <i>In Vitro</i> Study.\",\"authors\":\"Mehdi Moeil, Majid Tafrihi, Ehsan Nazifi, Maryam Radfar\",\"doi\":\"10.18502/ajmb.v17i2.18564\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Teucrium persicum</i> (<i>T. persicum</i>) is a well-known Iranian endemic plant that grows in the southern regions of Iran. It is used as a tea to treat abdominal pains, hyperlipidemia, and diabetes in traditional Iranian medicine. It has been previously found that the methanolic extract of <i>T. persicum</i> exerts significant cytotoxicity and inhibitory effects on different cancer cells. This study aimed to investigate the effects of ethyl acetate extract of <i>T. persicum</i> on MCF-7 cells.</p><p><strong>Methods: </strong>The experiments included MTT, DAPI staining, and investigating the expression of <i>BAX</i> and <i>BCL2</i> genes. The extract had a significant cytotoxic effect on MCF-7 cells, with an IC<sub>50</sub> value of 50 <i>μg/ml</i> for 48 <i>hr</i>.</p><p><strong>Results: </strong>DAPI staining assays showed that the extract induced morphological changes, chromatin condensation, and nuclear fragmentation. Additionally, the ethyl acetate extract induced the expression of <i>BAX</i> and down-regulated <i>BCL2</i> genes.</p><p><strong>Conclusion: </strong>These findings suggest that <i>T. persicum</i> has strong cytotoxic properties and warrants further investigation.</p>\",\"PeriodicalId\":8669,\"journal\":{\"name\":\"Avicenna journal of medical biotechnology\",\"volume\":\"17 2\",\"pages\":\"131-135\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123179/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Avicenna journal of medical biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ajmb.v17i2.18564\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna journal of medical biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ajmb.v17i2.18564","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
The Pro-Apoptosis Induction of Teucrium persicum Ethyl Acetate Extract on MCF-7 Cells: An In Vitro Study.
Background: Teucrium persicum (T. persicum) is a well-known Iranian endemic plant that grows in the southern regions of Iran. It is used as a tea to treat abdominal pains, hyperlipidemia, and diabetes in traditional Iranian medicine. It has been previously found that the methanolic extract of T. persicum exerts significant cytotoxicity and inhibitory effects on different cancer cells. This study aimed to investigate the effects of ethyl acetate extract of T. persicum on MCF-7 cells.
Methods: The experiments included MTT, DAPI staining, and investigating the expression of BAX and BCL2 genes. The extract had a significant cytotoxic effect on MCF-7 cells, with an IC50 value of 50 μg/ml for 48 hr.
Results: DAPI staining assays showed that the extract induced morphological changes, chromatin condensation, and nuclear fragmentation. Additionally, the ethyl acetate extract induced the expression of BAX and down-regulated BCL2 genes.
Conclusion: These findings suggest that T. persicum has strong cytotoxic properties and warrants further investigation.
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