为成功的蛋白质结晶实验做准备。

IF 1.1 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Acta crystallographica. Section F, Structural biology communications Pub Date : 2025-06-02 DOI:10.1107/S2053230X25004650

Gabrielle R. Budziszewski, Vivian Stojanoff, Sarah E. J. Bowman

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引用次数: 0

摘要

基于晶体的结构方法，包括x射线晶体学，经常用于确定生物分子的高分辨率结构。所有基于晶体的衍射方法首先需要制备生物分子晶体，精心制备结晶实验的样品可以增加成功的频率。在本文中，提出了优化影响结晶的因素的策略，其中缓冲液和还原剂最有利于结晶技术的使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Preparing for successful protein crystallization experiments

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Preparing for successful protein crystallization experiments

Crystal-based structural methods, including X-ray crystallography, are frequently utilized for the determination of high-resolution structures of biomolecules. All crystal-based diffraction methods first require the preparation of biomolecular crystals, and careful sample preparation for crystallization experiments can increase the frequency of success. In this article, strategies to optimize factors that can impact crystallization are presented, from which buffers and reducing agents are most favorable to which crystallization techniques could be used.

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来源期刊

Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.