肠道微生物群和血清代谢组学揭示了黄参多糖在改善衰老加速小鼠阿尔茨海默病症状中的作用。

IF 3.5 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Zhiyuan Zhang, Shuai Wang, Rong Rong, Guoying Zhang, Zheng Li, Yuanyuan Li, Rongxiang Wang, Yuhong Liu, Kejian Li
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引用次数: 0

摘要

阿尔茨海默病(AD)是一种复杂的神经退行性疾病,治疗方案有限,迫切需要针对其潜在机制的创新策略。蓖麻多糖(Phellinus ribis polysaccharides, PRG)是一种具有神经保护和微生物调节作用的生物活性化合物,有望通过调节肠-脑轴来解决AD病理。本研究旨在探讨PRG对衰老加速小鼠模型(SAMP8)肠道微生物群组成和血清代谢组学特征的影响,并揭示其缓解AD症状的潜在机制。采用16S rRNA基因测序分析PRG处理SAMP8小鼠肠道菌群组成。采用基于超高效液相色谱四极杆/静电场轨道阱高分辨率质谱技术的非靶向代谢组学分析血清样品中代谢物的变化。采用Spearman相关分析进一步探讨肠道菌群与血清代谢物之间的关系。PRG通过增加有益菌属的丰度,降低致病菌水平,恢复优势菌门的优势地位,显著改善SAMP8小鼠肠道生态失调。血清代谢组学分析显示,PRG干预导致ad相关代谢物发生显著变化,包括苯丙氨酸和氧化应激标志物。综合分析表明,肠道菌群变化与血清代谢物之间存在相关性。PRG可通过调节肠道菌群和血清代谢物,缓解衰老加速小鼠AD症状,为PRG作为AD的潜在治疗药物提供科学依据。本研究探讨了PRG干预下肠道菌群和血清代谢物在神经退行性疾病中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gut microbiota and serum metabolomics unveil the role of Phellinus ribis polysaccharides in improving Alzheimer's disease symptoms in senescence-accelerated mice.

Alzheimer's disease (AD), a complex neurodegenerative disorder with limited therapeutic options, urgently requires innovative strategies targeting its underlying mechanisms. Phellinus ribis polysaccharides (PRG), a bioactive compound with proven neuroprotective and microbiota-modulating effects, hold promise for addressing AD pathology through gut-brain axis regulation. This study aims to investigate the effects of PRG on the gut microbiota composition and serum metabolomic profile of a senescence-accelerated mouse model (SAMP8) and to reveal its potential mechanisms in alleviating symptoms of AD. The gut microbiota composition of SAMP8 mice treated with PRG was analyzed using 16S rRNA gene sequencing. Non-targeted metabolomics, based on ultra-performance liquid chromatography quadrupole/electrostatic field orbitrap high-resolution mass spectrometry, was employed to analyze changes in metabolites in the serum samples. Spearman correlation analysis was further used to explore the association between gut microbiota and serum metabolites. PRG significantly improved gut dysbiosis in SAMP8 mice by increasing the abundance of beneficial bacterial genera, reducing pathogenic bacteria levels, and restoring the dominance of advantageous bacterial phyla. Serum metabolomics analysis revealed that PRG intervention led to significant changes in AD-related metabolites, including phenylalanine and oxidative stress markers. Combined analysis indicated a correlation between changes in gut microbiota and serum metabolites. PRG can alleviate AD symptoms in senescence-accelerated mice by regulating gut microbiota and serum metabolites, providing scientific evidence for PRG as a potential therapeutic agent for AD. This study explores the role of gut microbiota and serum metabolites under PRG intervention in neurodegenerative diseases.

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来源期刊
Metabolic brain disease
Metabolic brain disease 医学-内分泌学与代谢
CiteScore
5.90
自引率
5.60%
发文量
248
审稿时长
6-12 weeks
期刊介绍: Metabolic Brain Disease serves as a forum for the publication of outstanding basic and clinical papers on all metabolic brain disease, including both human and animal studies. The journal publishes papers on the fundamental pathogenesis of these disorders and on related experimental and clinical techniques and methodologies. Metabolic Brain Disease is directed to physicians, neuroscientists, internists, psychiatrists, neurologists, pathologists, and others involved in the research and treatment of a broad range of metabolic brain disorders.
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