Hamadou Dicko, Jéremie Vidal-Dupiol, Denis Saulnier, Eric Tambutté, Alexander Venn, Sylvie Tambutté, Virginie Chamard, Julien Duboisset
{"title":"生物矿物中受激拉曼散射的三维碳酸钙多形成像。","authors":"Hamadou Dicko, Jéremie Vidal-Dupiol, Denis Saulnier, Eric Tambutté, Alexander Venn, Sylvie Tambutté, Virginie Chamard, Julien Duboisset","doi":"10.1039/d5fd00025d","DOIUrl":null,"url":null,"abstract":"<p><p>We present a highly sensitive coherent Raman microscopy approach, which allows for the tridimensional (3D) imaging of a series of carbonate polymorphs in marine organisms. CaCO<sub>3</sub> biomineralization occurs from the transformation of metastable amorphous precursors and other crystalline phases into a final crystalline phase. Understanding biomineralization pathways requires identifying this physico-chemical temporal sequence. Our approach exploits the different vibrational signatures of amorphous calcium carbonate, aragonite, calcite, Mg-calcite or hemi-hydrated calcium carbonate. This optical method enables the production of spatially and spectrally resolved images of the different compounds. When applied on the growing edge of post-mortem samples of both <i>Pinctada margaritifera</i> pearl oyster shell and <i>Stylophora pistillata</i> coral, it allows for inferring a temporal crystallisation sequence. We thus highlight the existence of intermediate crystalline phases, involving magnesian calcite or hemi-hydrated calcium carbonate, respectively.</p>","PeriodicalId":76,"journal":{"name":"Faraday Discussions","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D Calcium carbonate polymorphs imaging with stimulated Raman scattering in biominerals.\",\"authors\":\"Hamadou Dicko, Jéremie Vidal-Dupiol, Denis Saulnier, Eric Tambutté, Alexander Venn, Sylvie Tambutté, Virginie Chamard, Julien Duboisset\",\"doi\":\"10.1039/d5fd00025d\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We present a highly sensitive coherent Raman microscopy approach, which allows for the tridimensional (3D) imaging of a series of carbonate polymorphs in marine organisms. CaCO<sub>3</sub> biomineralization occurs from the transformation of metastable amorphous precursors and other crystalline phases into a final crystalline phase. Understanding biomineralization pathways requires identifying this physico-chemical temporal sequence. Our approach exploits the different vibrational signatures of amorphous calcium carbonate, aragonite, calcite, Mg-calcite or hemi-hydrated calcium carbonate. This optical method enables the production of spatially and spectrally resolved images of the different compounds. When applied on the growing edge of post-mortem samples of both <i>Pinctada margaritifera</i> pearl oyster shell and <i>Stylophora pistillata</i> coral, it allows for inferring a temporal crystallisation sequence. We thus highlight the existence of intermediate crystalline phases, involving magnesian calcite or hemi-hydrated calcium carbonate, respectively.</p>\",\"PeriodicalId\":76,\"journal\":{\"name\":\"Faraday Discussions\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Faraday Discussions\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d5fd00025d\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Faraday Discussions","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5fd00025d","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
3D Calcium carbonate polymorphs imaging with stimulated Raman scattering in biominerals.
We present a highly sensitive coherent Raman microscopy approach, which allows for the tridimensional (3D) imaging of a series of carbonate polymorphs in marine organisms. CaCO3 biomineralization occurs from the transformation of metastable amorphous precursors and other crystalline phases into a final crystalline phase. Understanding biomineralization pathways requires identifying this physico-chemical temporal sequence. Our approach exploits the different vibrational signatures of amorphous calcium carbonate, aragonite, calcite, Mg-calcite or hemi-hydrated calcium carbonate. This optical method enables the production of spatially and spectrally resolved images of the different compounds. When applied on the growing edge of post-mortem samples of both Pinctada margaritifera pearl oyster shell and Stylophora pistillata coral, it allows for inferring a temporal crystallisation sequence. We thus highlight the existence of intermediate crystalline phases, involving magnesian calcite or hemi-hydrated calcium carbonate, respectively.