Mahathir Mohammad, Fahmida Tasnim Richi, Rabiul Hossain, Md Arafat, Pair Ahmed Jiko, Nazim Uddin Emon, Sayed Al Hossain Rabbi, Tirtha Khastagir, Md Hemayet Hossain, Safaet Alam
{"title":"芋菜（Colocasia esculenta）的保肝实验Schott)花利用动物模型减轻氧化应激。","authors":"Mahathir Mohammad, Fahmida Tasnim Richi, Rabiul Hossain, Md Arafat, Pair Ahmed Jiko, Nazim Uddin Emon, Sayed Al Hossain Rabbi, Tirtha Khastagir, Md Hemayet Hossain, Safaet Alam","doi":"10.1002/ame2.70031","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Colocasia esculenta (L.) Schott, known as the taro vegetable, possesses various beneficial effects and is traditionally used in folk medicine. This study explores the ameliorative antioxidant and hepatoprotective effect of a methanolic extract of the C. esculenta flower (ME-CEF) against oxidative damage and hepatotoxicity in mice.</p><p><strong>Methods: </strong>The antioxidant efficacy of ME-CEF was assessed using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The hepatoprotective effect was investigated by an assessment of liver injury indicators (amino transferase [ALT], aspartate amino transferase [AST], alkaline phosphatase [ALP], bilirubin, creatinine) and normalizing lipid profiles (cholesterol [CHO], triglyceride [TG], high-density lipoprotein [HDL], and low-density lipoprotein [LDL]) along with histopathological study and antioxidant enzymes (CAT). A phytochemical analysis, both qualitative and quantitative, was conducted, including gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis and an in silico molecular docking study.</p><p><strong>Results: </strong>The Result Showed that ME-CEF Possesses Moderate ABTS and DPPH Scavenging Activity with IC<sub>50</sub> Values of 117.18 and 160.41 μg/mL. As Illustrated by Reducing Liver Enzymes (ALT, AST, ALP, Bilirubin, Creatinine) and Lipid Profile (CHO, TG, LDL) and Raising HDL Levels (p < 0.01), ME-CEF Dose Dependently Mitigated CCl<sub>4</sub>-Induced Acute Liver Injury. Furthermore, ME-CEF Blocked Hepatic Oxidative Stress by Boosting Antioxidant Enzymes (CAT) and Preventing Liver Tissue Damage and Apoptosis. In Silico Investigations Also Showed a Promising Binding Affinity with Tumor Necrosis Factor α (TNF-α), Interleukin 6 (IL-6), PRAP-1, and Xanthin Oxidoreductase, which Displayed Antioxidant and Hepatoprotective Candidacy while Notable Safety and Efficacy Profile Was Also Documented through ADME/T Studies. Histopathological Analysis Showed Reduced Hepatocellular Necrosis and Vascular Congestion in Silymarin and Extract Groups.</p><p><strong>Conclusion: </strong>Based on these results, our findings strongly recommend the medicinal use of the plant, highlighting its antioxidant and hepatoprotective potentials.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A hepatoprotective experiment on taro vegetable (Colocasia esculenta (L.) Schott) flower employing animal models by mitigating oxidative stress.\",\"authors\":\"Mahathir Mohammad, Fahmida Tasnim Richi, Rabiul Hossain, Md Arafat, Pair Ahmed Jiko, Nazim Uddin Emon, Sayed Al Hossain Rabbi, Tirtha Khastagir, Md Hemayet Hossain, Safaet Alam\",\"doi\":\"10.1002/ame2.70031\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Colocasia esculenta (L.) Schott, known as the taro vegetable, possesses various beneficial effects and is traditionally used in folk medicine. This study explores the ameliorative antioxidant and hepatoprotective effect of a methanolic extract of the C. esculenta flower (ME-CEF) against oxidative damage and hepatotoxicity in mice.</p><p><strong>Methods: </strong>The antioxidant efficacy of ME-CEF was assessed using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The hepatoprotective effect was investigated by an assessment of liver injury indicators (amino transferase [ALT], aspartate amino transferase [AST], alkaline phosphatase [ALP], bilirubin, creatinine) and normalizing lipid profiles (cholesterol [CHO], triglyceride [TG], high-density lipoprotein [HDL], and low-density lipoprotein [LDL]) along with histopathological study and antioxidant enzymes (CAT). A phytochemical analysis, both qualitative and quantitative, was conducted, including gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis and an in silico molecular docking study.</p><p><strong>Results: </strong>The Result Showed that ME-CEF Possesses Moderate ABTS and DPPH Scavenging Activity with IC<sub>50</sub> Values of 117.18 and 160.41 μg/mL. As Illustrated by Reducing Liver Enzymes (ALT, AST, ALP, Bilirubin, Creatinine) and Lipid Profile (CHO, TG, LDL) and Raising HDL Levels (p < 0.01), ME-CEF Dose Dependently Mitigated CCl<sub>4</sub>-Induced Acute Liver Injury. Furthermore, ME-CEF Blocked Hepatic Oxidative Stress by Boosting Antioxidant Enzymes (CAT) and Preventing Liver Tissue Damage and Apoptosis. In Silico Investigations Also Showed a Promising Binding Affinity with Tumor Necrosis Factor α (TNF-α), Interleukin 6 (IL-6), PRAP-1, and Xanthin Oxidoreductase, which Displayed Antioxidant and Hepatoprotective Candidacy while Notable Safety and Efficacy Profile Was Also Documented through ADME/T Studies. Histopathological Analysis Showed Reduced Hepatocellular Necrosis and Vascular Congestion in Silymarin and Extract Groups.</p><p><strong>Conclusion: </strong>Based on these results, our findings strongly recommend the medicinal use of the plant, highlighting its antioxidant and hepatoprotective potentials.</p>\",\"PeriodicalId\":93869,\"journal\":{\"name\":\"Animal models and experimental medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal models and experimental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/ame2.70031\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Health Professions\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal models and experimental medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/ame2.70031","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}
A hepatoprotective experiment on taro vegetable (Colocasia esculenta (L.) Schott) flower employing animal models by mitigating oxidative stress.
Background: Colocasia esculenta (L.) Schott, known as the taro vegetable, possesses various beneficial effects and is traditionally used in folk medicine. This study explores the ameliorative antioxidant and hepatoprotective effect of a methanolic extract of the C. esculenta flower (ME-CEF) against oxidative damage and hepatotoxicity in mice.
Methods: The antioxidant efficacy of ME-CEF was assessed using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The hepatoprotective effect was investigated by an assessment of liver injury indicators (amino transferase [ALT], aspartate amino transferase [AST], alkaline phosphatase [ALP], bilirubin, creatinine) and normalizing lipid profiles (cholesterol [CHO], triglyceride [TG], high-density lipoprotein [HDL], and low-density lipoprotein [LDL]) along with histopathological study and antioxidant enzymes (CAT). A phytochemical analysis, both qualitative and quantitative, was conducted, including gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis and an in silico molecular docking study.
Results: The Result Showed that ME-CEF Possesses Moderate ABTS and DPPH Scavenging Activity with IC50 Values of 117.18 and 160.41 μg/mL. As Illustrated by Reducing Liver Enzymes (ALT, AST, ALP, Bilirubin, Creatinine) and Lipid Profile (CHO, TG, LDL) and Raising HDL Levels (p < 0.01), ME-CEF Dose Dependently Mitigated CCl4-Induced Acute Liver Injury. Furthermore, ME-CEF Blocked Hepatic Oxidative Stress by Boosting Antioxidant Enzymes (CAT) and Preventing Liver Tissue Damage and Apoptosis. In Silico Investigations Also Showed a Promising Binding Affinity with Tumor Necrosis Factor α (TNF-α), Interleukin 6 (IL-6), PRAP-1, and Xanthin Oxidoreductase, which Displayed Antioxidant and Hepatoprotective Candidacy while Notable Safety and Efficacy Profile Was Also Documented through ADME/T Studies. Histopathological Analysis Showed Reduced Hepatocellular Necrosis and Vascular Congestion in Silymarin and Extract Groups.
Conclusion: Based on these results, our findings strongly recommend the medicinal use of the plant, highlighting its antioxidant and hepatoprotective potentials.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
