Kelly DeWeese, Gary Molano, Sara Calhoun, Anna Lipzen, Jerry Jenkins, Melissa Williams, Christopher Plott, Jayson Talag, Jane Grimwood, Jean-Luc Jannink, Igor V Grigoriev, Jeremy Schmutz, Charles Yarish, Sergey Nuzhdin, Scott Lindell
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Cultivation of <i>Saccharina latissima</i> for human consumption has increased significantly this century in both North America and Europe, and its single blade morphology allows for dense seeding practices used in the cultivation of its Asian sister species, <i>Saccharina japonica</i>. While <i>Saccharina latissima</i> has potential as a human food crop, insufficient information from genetic resources has limited molecular breeding in sugar kelp aquaculture. We present scaffolded and annotated <i>Saccharina latissima</i> nuclear and organelle genomes from a female gametophyte collected from Black Ledge, Groton, Connecticut. This <i>Saccharina latissima</i> genome compares well with other published kelp genomes and contains 218 scaffolds with a scaffold N50 of 1.35 Mb, a GC content of 49.84%, and 25,012 predicted genes. We also validated this genome by comparing the synteny and completeness of this <i>Saccharina latissima</i> genome to other kelp genomes. Our team has successfully performed initial genomic selection trials with sugar kelp using a draft version of this genome. This <i>Saccharina latissima</i> genome expands the genetic toolkit for the economically and ecologically important sugar kelp and will be a fundamental resource for future foundational science, breeding, and conservation efforts.</p>","PeriodicalId":12750,"journal":{"name":"Frontiers in Genetics","volume":"16 ","pages":"1494480"},"PeriodicalIF":2.8000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116465/pdf/","citationCount":"0","resultStr":"{\"title\":\"Scaffolded and annotated nuclear and organelle genomes of the North American brown alga <i>Saccharina latissima</i>.\",\"authors\":\"Kelly DeWeese, Gary Molano, Sara Calhoun, Anna Lipzen, Jerry Jenkins, Melissa Williams, Christopher Plott, Jayson Talag, Jane Grimwood, Jean-Luc Jannink, Igor V Grigoriev, Jeremy Schmutz, Charles Yarish, Sergey Nuzhdin, Scott Lindell\",\"doi\":\"10.3389/fgene.2025.1494480\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Increasing the genomic resources of emerging aquaculture crop targets can expedite breeding processes as seen in molecular breeding advances in agriculture. 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Scaffolded and annotated nuclear and organelle genomes of the North American brown alga Saccharina latissima.
Increasing the genomic resources of emerging aquaculture crop targets can expedite breeding processes as seen in molecular breeding advances in agriculture. High quality annotated reference genomes are essential to implement this relatively new molecular breeding scheme and benefit research areas such as population genetics, gene discovery, and gene mechanics by providing a tool for standard comparison. The brown macroalga Saccharina latissima (sugar kelp) is an ecologically and economically important kelp that is found in both the northern Pacific and Atlantic Oceans. Cultivation of Saccharina latissima for human consumption has increased significantly this century in both North America and Europe, and its single blade morphology allows for dense seeding practices used in the cultivation of its Asian sister species, Saccharina japonica. While Saccharina latissima has potential as a human food crop, insufficient information from genetic resources has limited molecular breeding in sugar kelp aquaculture. We present scaffolded and annotated Saccharina latissima nuclear and organelle genomes from a female gametophyte collected from Black Ledge, Groton, Connecticut. This Saccharina latissima genome compares well with other published kelp genomes and contains 218 scaffolds with a scaffold N50 of 1.35 Mb, a GC content of 49.84%, and 25,012 predicted genes. We also validated this genome by comparing the synteny and completeness of this Saccharina latissima genome to other kelp genomes. Our team has successfully performed initial genomic selection trials with sugar kelp using a draft version of this genome. This Saccharina latissima genome expands the genetic toolkit for the economically and ecologically important sugar kelp and will be a fundamental resource for future foundational science, breeding, and conservation efforts.
Frontiers in GeneticsBiochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
5.50
自引率
8.10%
发文量
3491
审稿时长
14 weeks
期刊介绍:
Frontiers in Genetics publishes rigorously peer-reviewed research on genes and genomes relating to all the domains of life, from humans to plants to livestock and other model organisms. Led by an outstanding Editorial Board of the world’s leading experts, this multidisciplinary, open-access journal is at the forefront of communicating cutting-edge research to researchers, academics, clinicians, policy makers and the public.
The study of inheritance and the impact of the genome on various biological processes is well documented. However, the majority of discoveries are still to come. A new era is seeing major developments in the function and variability of the genome, the use of genetic and genomic tools and the analysis of the genetic basis of various biological phenomena.