通过内着丝点的力传递通过着丝点DNA序列增强。

IF 6.4 1区生物学 Q1 BIOLOGY

eLife Pub Date : 2025-05-29 DOI:10.7554/eLife.105150

Elise Miedlar, Grace E Hamilton, Samuel R Witus, Sara J Gonske, Michael Riffle, Alex Zelter, Rachel E Klevit, Charles L Asbury, Yoana N Dimitrova, Trisha N Davis

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引用次数: 0

摘要

此前，我们利用重组酿酒酵母蛋白重组了一个最小的功能着丝点，该着丝点能够将力从动态微管传递到含有着丝粒特异性组蛋白变体Cse4的核小体（Hamilton等，2020）。这项工作揭示了通过内部着丝点的两条力传递途径：通过Mif2和通过Okp1/Ame1复合体（OA）。在这里，我们使用含有出芽酵母点着丝粒的关键着丝粒决定元件的嵌合DNA序列，证明了在含有cse4的核小体中存在着丝粒DNA序列可显著增强oa介导的联系。我们的研究结果表明，着丝粒序列对于微管力到染色体的传递是重要的。

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Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences.

Previously, we reconstituted a minimal functional kinetochore from recombinant Saccharomyces cerevisiae proteins that was capable of transmitting force from dynamic microtubules to nucleosomes containing the centromere-specific histone variant Cse4 (Hamilton et al., 2020). This work revealed two paths of force transmission through the inner kinetochore: through Mif2 and through the Okp1/Ame1 complex (OA). Here, using a chimeric DNA sequence that contains crucial centromere-determining elements of the budding yeast point centromere, we demonstrate that the presence of centromeric DNA sequences in Cse4-containing nucleosomes significantly strengthens OA-mediated linkages. Our findings indicate that centromeric sequences are important for the transmission of microtubule-based forces to the chromosome.

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来源期刊

eLife BIOLOGY-

CiteScore

12.90

自引率

3.90%

发文量

3122

审稿时长

17 weeks

期刊介绍： eLife is a distinguished, not-for-profit, peer-reviewed open access scientific journal that specializes in the fields of biomedical and life sciences. eLife is known for its selective publication process, which includes a variety of article types such as: Research Articles: Detailed reports of original research findings. Short Reports: Concise presentations of significant findings that do not warrant a full-length research article. Tools and Resources: Descriptions of new tools, technologies, or resources that facilitate scientific research. Research Advances: Brief reports on significant scientific advancements that have immediate implications for the field. Scientific Correspondence: Short communications that comment on or provide additional information related to published articles. Review Articles: Comprehensive overviews of a specific topic or field within the life sciences.